PMID- 17035097
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20210221
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 146
IP  - 1-2
DP  - 2007 Jul-Aug
TI  - The yeast genome may harbor hypoxia response elements (HRE).
PG  - 255-263
LID - S1532-0456(06)00200-6 [pii]
LID - 10.1016/j.cbpc.2006.08.013 [doi]
AB  - The hypoxia-inducible factor-1 (HIF-1) is a heterodimeric transcription factor 
      activated when cells are submitted to hypoxia. The heterodimer is composed of two 
      subunits, HIF-1alpha and the constitutively expressed HIF-1beta. During normoxia, 
      HIF-1alpha is degraded by the 26S proteasome, but hypoxia causes HIF-1alpha to be 
      stabilized, enter the nucleus and bind to HIF-1beta, thus forming the active 
      complex. The complex then binds to the regulatory sequences of various genes 
      involved in physiological and pathological processes. The specific regulatory 
      sequence recognized by HIF-1 is the hypoxia response element (HRE) that has the 
      consensus sequence 5'BRCGTGVBBB3'. Although the basic transcriptional regulation 
      machinery is conserved between yeast and mammals, Saccharomyces cerevisiae does 
      not express HIF-1 subunits. However, we hypothesized that baker's yeast has a 
      protein analogous to HIF-1 which participates in the response to changes in 
      oxygen levels by binding to HRE sequences. In this study we screened the yeast 
      genome for HREs using probabilistic motif search tools. We described 24 yeast 
      genes containing motifs with high probability of being HREs (p-value<0.1) and 
      classified them according to biological function. Our results show that S. 
      cerevisiae may harbor HREs and indicate that a transcription factor analogous to 
      HIF-1 may exist in this organism.
FAU - Ferreira, Tulio Cesar
AU  - Ferreira TC
AD  - Laboratorio de Biologia Molecular, Departamento de Biologia Celular, Instituto de 
      Ciencias Biologicas, Universidade de Brasilia, Brasilia, DF, 70910-900, Brazil.
FAU - Hertzberg, Libi
AU  - Hertzberg L
AD  - Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel; Department of 
      Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel.
FAU - Gassmann, Max
AU  - Gassmann M
AD  - Institute of Veterinary Physiology, Vetsuisse Faculty and Zurich Center for 
      Integrative Human Physiology (ZIHP), University of Zurich, Winterthurerstrasse 
      260, CH-8057, Zurich, Switzerland.
FAU - Campos, Elida Geralda
AU  - Campos EG
AD  - Laboratorio de Biologia Molecular, Departamento de Biologia Celular, Instituto de 
      Ciencias Biologicas, Universidade de Brasilia, Brasilia, DF, 70910-900, Brazil. 
      Electronic address: elida@unb.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060906
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Base Sequence
MH  - *Genome
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Proteasome Endopeptidase Complex/genetics/metabolism
MH  - Response Elements/*genetics
MH  - Saccharomyces cerevisiae/*genetics
EDAT- 2006/10/13 09:00
MHDA- 2007/10/03 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/05/08 00:00 [received]
PHST- 2006/08/22 00:00 [revised]
PHST- 2006/08/28 00:00 [accepted]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - S1532-0456(06)00200-6 [pii]
AID - 10.1016/j.cbpc.2006.08.013 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2007 Jul-Aug;146(1-2):255-263. doi: 
      10.1016/j.cbpc.2006.08.013. Epub 2006 Sep 6.