PMID- 17035346
OWN - NLM
STAT- MEDLINE
DCOM- 20070122
LR  - 20111117
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 18
IP  - 12
DP  - 2006 Dec
TI  - Efficiency of peptide presentation by dendritic cells compared with other cell 
      types: implications for cross-priming.
PG  - 1647-54
AB  - Dendritic cells (DCs) play a key role in the induction of cellular immune 
      responses by harvesting antigens from peripheral tissue for cross-priming CD8(+) 
      T cells. It has been demonstrated that apoptotic bodies, whole- or 
      degraded-cell-associated or soluble antigens as well as heat shock protein-bound 
      peptides can be taken up, processed and cross-presented by DCs. Since cells are 
      continuously releasing peptides from their surface MHC molecules, DCs in the 
      tissues are exposed to such peptides and might process and present them to T 
      cells as an additional pathway for cross-priming. To investigate this 
      possibility, we compared and characterized the presentation of exogenous peptides 
      by DCs and other cell types employing novel recombinant antibodies with TCR-like 
      specificities for specific peptide-MHC complexes (pMHCs). These analyses reveal 
      that loading of immature and mature DCs with peptide is far less efficient than 
      it is for monocytes, T and B lymphocytes, B-lymphoblastoid, melanoma and 
      TAP-deficient T2 cells. This inefficiency of peptide transfer to the MHC 
      molecules of DCs makes it unlikely that these cells recycle peptides released 
      from the MHC molecules of other cells and may explain why cross-presentation of 
      such peptides has not yet been observed.
FAU - Zehn, Dietmar
AU  - Zehn D
AD  - Department of Dermatology, Charite, University Medicine Berlin, Humboldt 
      University, Schumannstrasse 20/21, D-10117 Berlin, Germany.
FAU - Cohen, Cyril J
AU  - Cohen CJ
FAU - Reiter, Yoram
AU  - Reiter Y
FAU - Walden, Peter
AU  - Walden P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061011
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Antigen Presentation
MH  - B-Lymphocytes/immunology
MH  - Cell Line, Tumor
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/*immunology
MH  - HLA-A Antigens
MH  - HLA-A2 Antigen
MH  - Humans
MH  - Major Histocompatibility Complex
MH  - Melanoma/immunology
MH  - Molecular Sequence Data
MH  - Monocytes/immunology
MH  - Peptides/chemical synthesis/chemistry/*immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2006/10/13 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - dxl098 [pii]
AID - 10.1093/intimm/dxl098 [doi]
PST - ppublish
SO  - Int Immunol. 2006 Dec;18(12):1647-54. doi: 10.1093/intimm/dxl098. Epub 2006 Oct 
      11.