PMID- 17035709
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20061130
IS  - 0014-312X (Print)
IS  - 0014-312X (Linking)
VI  - 38
IP  - 6
DP  - 2006
TI  - F344 rat liver nonparenchymal cell transplantation can increase the number of 
      albumin-positive hepatocytes in the liver following hematopoietic reconstitution 
      in irradiated analbuminemic rats.
PG  - 533-9
AB  - The adult liver contains hematopoietic stem cells that can reconstitute the bone 
      marrow. We tested whether bone marrow cells (BMCs) derived from liver 
      nonparenchymal cells (LNPCs) can increase the number of hepatocytes within 
      livers. LNPCs from Fischer 344 rats (F344) were infused into the penile veins of 
      F344 congenic Nagase's analbuminenic rats (F344alb) immediately after whole-body 
      irradiation, and the recipients were sacrificed 8 weeks later. Eleven of 15 
      (73.3%) F344alb that received the LNPC transplantation after irradiation 
      survived, while only 1 of 8 (12.5%) F344alb that received irradiation alone was 
      alive after 8 weeks. Normal albumin gene sequences were detected by PCR in BMCs 
      of the recipient F344alb that received LNPC transplantation after irradiation, 
      indicating that F344alb bone marrow was reconstituted by F344 LNPCs. Although 
      single or pairs of albumin-positive (Alb+) hepatocytes were seen in the liver of 
      untreated F344alb and those with irradiation or LNPC transplantation alone, 
      clusters consisting of >3 Alb+ hepatocytes were detected in the livers of F344alb 
      with the LNPC or BMC transplantation after irradiation together with single or 
      double Alb+ cells. Normal albumin gene sequences were detected by PCR in the DNA 
      isolated from such Alb+ hepatocyte clusters microdissected from the immunostained 
      sections. The data indicate that BMCs derived from F344 LNPCs could increase the 
      number Alb+ hepatocytes within the F344alb liver.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Xia, Yan
AU  - Xia Y
AD  - Department of Surgery, Asahikawa Medical College, Asahikawa, Japan.
FAU - Zhang, Biao
AU  - Zhang B
FAU - Inagaki, Mitsuhiro
AU  - Inagaki M
FAU - Arikura, Jun
AU  - Arikura J
FAU - Miyakoshi, Masaaki
AU  - Miyakoshi M
FAU - Yoshie, Masumi
AU  - Yoshie M
FAU - Ogawa, Katsuhiro
AU  - Ogawa K
FAU - Kasai, Shinichi
AU  - Kasai S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061011
PL  - Switzerland
TA  - Eur Surg Res
JT  - European surgical research. Europaische chirurgische Forschung. Recherches 
      chirurgicales europeennes
JID - 0174752
RN  - 0 (Albumins)
SB  - IM
MH  - Albumins/analysis/*physiology
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - *Hematopoiesis
MH  - Hepatocytes/chemistry/*cytology
MH  - Liver/*cytology
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - *Stem Cell Transplantation
MH  - Whole-Body Irradiation
EDAT- 2006/10/13 09:00
MHDA- 2006/12/23 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/05/20 00:00 [received]
PHST- 2006/07/20 00:00 [accepted]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - 96162 [pii]
AID - 10.1159/000096162 [doi]
PST - ppublish
SO  - Eur Surg Res. 2006;38(6):533-9. doi: 10.1159/000096162. Epub 2006 Oct 11.