PMID- 17036078
OWN - NLM
STAT- MEDLINE
DCOM- 20070125
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 8
DP  - 2006 Aug
TI  - Identification of chromosomal fusion sites in Arabidopsis mutants using 
      sequential bicolour BAC-FISH.
PG  - 1036-42
AB  - Double stranded chromosomal breaks are repaired by homologous recombination or 
      nonhomologous end joining (NHEJ). When broken chromosome ends are fused together 
      by NHEJ, the resulting dicentric chromosomes can be detected as anaphase bridges 
      during the subsequent mitosis. Telomeres in the absence of functional telomerase 
      shorten, became unprotected, and are eventually recognized by the cell repair 
      system as double stranded breaks. As result, chromosomes of Arabidopsis thaliana 
      plants that are deficient in the gene for telomerase reverse transcriptase (TERT) 
      are prone to chromosome fusions. We use Arabidopsis tert-/- mutants as a model 
      system for analyzing terminal chromosome fusions. Here we report a novel and 
      sensitive cytogenetic assay for the identification and characterization of 
      chromosome-terminal fusion events by employing fluorescence in situ hybridization 
      (FISH) with multiple probes and a repeated hybridization approach. A mixture of 
      chromosome-specific subtelomeric probes is applied successively in 3 FISH 
      reactions to the slides containing mitotic anaphase figures with anaphase 
      bridges. Each figure is registered by a CCD camera after each in situ 
      hybridization procedure. By comparing the signals presented on the bridge in 
      successive images the assessment of the particular chromosome arms involved in 
      fusion is possible. This experimental setup enables unambiguous identification of 
      individual chromosome ends employed in fusion events.
FAU - Mokros, Petr
AU  - Mokros P
AD  - Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy 
      Sciences of the Czech Republic, Brno, Czech Republic.
FAU - Vrbsky, Jan
AU  - Vrbsky J
FAU - Siroky, Jiri
AU  - Siroky J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Arabidopsis/enzymology/*genetics
MH  - Chromosome Breakage
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Plant/genetics
MH  - Gene Fusion
MH  - Genes, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Mutation
MH  - Telomerase/genetics
EDAT- 2006/10/13 09:00
MHDA- 2007/01/26 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/01/26 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - g06-082 [pii]
AID - 10.1139/g06-082 [doi]
PST - ppublish
SO  - Genome. 2006 Aug;49(8):1036-42. doi: 10.1139/g06-082.