PMID- 1703618
OWN - NLM
STAT- MEDLINE
DCOM- 19910306
LR  - 20190610
IS  - 0140-6736 (Print)
IS  - 0140-6736 (Linking)
VI  - 337
IP  - 8737
DP  - 1991 Feb 9
TI  - Improved detection of rotavirus shedding by polymerase chain reaction.
PG  - 323-6
AB  - To improve identification of children excreting rotavirus a method for the 
      amplification of rotavirus RNA by the polymerase chain reaction (PCR) was 
      developed. The assay was compared with a solid-phase enzyme immunoassay in the 
      detection of rotavirus shedding by infants in hospital during the winter peak of 
      rotavirus infections. Forty children were studied in an intermediate care unit 
      after transfer from intensive care units. Only two were admitted primarily 
      because of diarrhoea; the other thirty-eight were admitted for management of 
      various other disorders. Rotavirus shedding was detected by enzyme immunoassay in 
      twenty of the infants, and nine of these (aged 1 week to 8 months) remained in 
      hospital for more than 5 days after the initial detection of rotavirus and could 
      be studied long term. Of 103 faecal samples from the nine infants, 60 (58%) 
      contained rotavirus RNA detected by reverse-transcriptase (RT)/PCR, whereas only 
      37 (36%) were positive for rotavirus antigen by the immunoassay (chi 2 = 10.3, p 
      less than 0.002). The geometric mean time of rotavirus shedding was 9.5 (range 
      1-19) days as detected by RT/PCR and 5.7 (range 1-17) days by the immunoassay (p 
      less than 0.018). In five of the nine children, RT/PCR detected rotavirus 
      shedding for 2-7 days longer than the immunoassay and in four children RT/PCR was 
      positive 1 or more days before rotavirus antigen was detected. Further studies 
      should attempt to find out whether infected infants are capable of spreading 
      wild-type virus during periods when they are not shedding antigen as detectable 
      by enzyme immunoassay.
FAU - Wilde, J
AU  - Wilde J
AD  - Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, Maryland 
      21205.
FAU - Yolken, R
AU  - Yolken R
FAU - Willoughby, R
AU  - Willoughby R
FAU - Eiden, J
AU  - Eiden J
LA  - eng
GR  - R01 DK 33089/DK/NIDDK NIH HHS/United States
GR  - R29 AI 24922/AI/NIAID NIH HHS/United States
GR  - U01 AI 30420/AI/NIAID NIH HHS/United States
PT  - Clinical Trial
PT  - Comparative Study
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Lancet
JT  - Lancet (London, England)
JID - 2985213R
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Antigens, Viral/*analysis
MH  - Cross Infection/enzymology/*prevention & control
MH  - Evaluation Studies as Topic
MH  - Feces/microbiology
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Polymerase Chain Reaction/methods/standards
MH  - Prospective Studies
MH  - RNA, Viral/*analysis
MH  - RNA-Directed DNA Polymerase/analysis
MH  - Rotavirus/genetics/immunology/*physiology
MH  - Rotavirus Infections/enzymology/*prevention & control
MH  - Seasons
MH  - Virus Replication/*physiology
EDAT- 1991/02/09 00:00
MHDA- 1991/02/09 00:01
CRDT- 1991/02/09 00:00
PHST- 1991/02/09 00:00 [pubmed]
PHST- 1991/02/09 00:01 [medline]
PHST- 1991/02/09 00:00 [entrez]
AID - 0140-6736(91)90945-L [pii]
AID - 10.1016/0140-6736(91)90945-l [doi]
PST - ppublish
SO  - Lancet. 1991 Feb 9;337(8737):323-6. doi: 10.1016/0140-6736(91)90945-l.