PMID- 17036397
OWN - NLM
STAT- MEDLINE
DCOM- 20061205
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 12
IP  - 38
DP  - 2006 Oct 14
TI  - Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and 
      increased expression in individuals from northern Brazil with gastric 
      adenocarcinoma.
PG  - 6207-11
AB  - AIM: To investigate chromosome 8 numerical aberrations, C-MYC oncogene 
      alterations and its expression in gastric cancer and to correlate these findings 
      with histopathological characteristics of gastric tumors. METHODS: Specimens were 
      collected surgically from seven patients with gastric adenocarcinomas. 
      Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) 
      for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases 
      showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and 
      intestinal histopathological types of Lauren. No significant difference (P < 
      0.05) was observed between the level of chromosome 8 ploidy and the site, stage 
      or histological type of the adenocarcinomas. C-MYC high amplification, like 
      homogeneously stained regions (HSRs) and double minutes (DMs), was observed only 
      in the intestinal-type. Structural rearrangement of C-MYC, like translocation, 
      was observed only in the diffuse type. Regarding C-MYC gene, a significant 
      difference (P < 0.05) was observed between the two histological types. The C-MYC 
      protein was expressed in all the studied cases. In the intestinal-type the C-MYC 
      immunoreactivity was localized only in the nucleus and in the diffuse type in the 
      nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between 
      intestinal and diffuse types of gastric tumors support the hypothesis that these 
      types follow different genetic pathways.
FAU - Calcagno, Danielle-Queiroz
AU  - Calcagno DQ
AD  - Human Cytogenetics and Toxicological Genetics Laboratory, Department of Biology, 
      Center of Biological Sciences, Federal University of Para, Belem, PA, Brazil.
FAU - Leal, Mariana-Ferreira
AU  - Leal MF
FAU - Seabra, Aline-Damaceno
AU  - Seabra AD
FAU - Khayat, Andre-Salim
AU  - Khayat AS
FAU - Chen, Elizabeth Suchi
AU  - Chen ES
FAU - Demachki, Samia
AU  - Demachki S
FAU - Assumpcao, Paulo Pimentel
AU  - Assumpcao PP
FAU - Faria, Mario Henrique Girao
AU  - Faria MH
FAU - Rabenhorst, Silvia Helena Barem
AU  - Rabenhorst SH
FAU - Ferreira, Marcia Valeria Pitombeira
AU  - Ferreira MV
FAU - de Arruda Cardoso Smith, Marilia
AU  - de Arruda Cardoso Smith M
FAU - Burbano, Rommel-Rodriguez
AU  - Burbano RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Brazil
MH  - *Chromosomes, Human, Pair 8
MH  - Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - *Genes, myc
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Stomach Neoplasms/*genetics
PMC - PMC4088119
EDAT- 2006/10/13 09:00
MHDA- 2006/12/09 09:00
PMCR- 2006/10/14
CRDT- 2006/10/13 09:00
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
PHST- 2006/10/14 00:00 [pmc-release]
AID - 10.3748/wjg.v12.i38.6207 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2006 Oct 14;12(38):6207-11. doi: 10.3748/wjg.v12.i38.6207.