PMID- 17041145
OWN - NLM
STAT- MEDLINE
DCOM- 20070122
LR  - 20221207
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 18
IP  - 12
DP  - 2006 Dec
TI  - The anti-microbial peptide LL-37 inhibits the activation of dendritic cells by 
      TLR ligands.
PG  - 1729-36
AB  - The endogenous anti-microbial peptide LL-37/hCAP-18 is an effector molecule of 
      the innate host defense system at surfaces of the body. Besides its direct 
      anti-microbial activity, the peptide interacts with different cell types. 
      Dendritic cells (DCs) play a central role in mucosal host defense. It was the aim 
      of the study to determine whether LL-37 modulates the response of DCs to 
      pathogen-associated molecular patterns. Monocyte-derived DCs were stimulated with 
      the Toll-like receptors (TLRs) ligands LPS, lipoteichoic acid and flagellin. We 
      measured classical markers of DC maturation and assayed the ability of the DCs to 
      activate T cell responses. Co-incubation with LL-37 resulted in suppressed 
      activation of DCs. Levels of released IL-6, IL-12p70 and TNF-alpha and surface 
      expression of HLA-DR, CD80, CD83, CD86 and the chemokine receptor CCR7 were 
      decreased. Exposure of DCs to LL-37 during LPS exposure induced co-cultured naive 
      T cells to produce less IL-2 and IFN-gamma and decreased their proliferation. The 
      response of memory T cells to a recall antigen was also decreased. In conclusion, 
      we demonstrate that the anti-microbial peptide LL-37 inhibits the activation of 
      DCs by TLR ligands. We propose that LL-37 is a regulator of host defense 
      responses at the intersection of innate and adaptive immune systems.
FAU - Kandler, Kerstin
AU  - Kandler K
AD  - Department of Internal Medicine, Division for Pulmonary Diseases, 
      Baldingerstrasse 1, 35043 Marburg, Germany.
FAU - Shaykhiev, Renat
AU  - Shaykhiev R
FAU - Kleemann, Peter
AU  - Kleemann P
FAU - Klescz, Frank
AU  - Klescz F
FAU - Lohoff, Michael
AU  - Lohoff M
FAU - Vogelmeier, Claus
AU  - Vogelmeier C
FAU - Bals, Robert
AU  - Bals R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061013
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Cathelicidins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antimicrobial Cationic Peptides/chemistry/*immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/*immunology
MH  - Humans
MH  - *Ligands
MH  - Lipopolysaccharides/immunology
MH  - Molecular Sequence Data
MH  - Monocytes/cytology/immunology
MH  - T-Lymphocytes/immunology
MH  - Toll-Like Receptors/*metabolism
MH  - Cathelicidins
EDAT- 2006/10/17 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/10/17 09:00
PHST- 2006/10/17 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/10/17 09:00 [entrez]
AID - dxl107 [pii]
AID - 10.1093/intimm/dxl107 [doi]
PST - ppublish
SO  - Int Immunol. 2006 Dec;18(12):1729-36. doi: 10.1093/intimm/dxl107. Epub 2006 Oct 
      13.