PMID- 1704226
OWN - NLM
STAT- MEDLINE
DCOM- 19910308
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 174
IP  - 2
DP  - 1991 Jan 31
TI  - Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its 
      expression in rat spleen cells and tumor cell lines.
PG  - 504-9
AB  - Rat monocyte chemoattractant protein-1 (MCP-1) cDNA was cloned. A cDNA library 
      was constructed from mRNA extracted from Con A-stimulated rat spleen cells. After 
      2 rounds of screening with a radiolabeled oligonucleotide probe and DNA 
      sequencing from plasmid DNAs, one clone which encoded for MCP-1 was obtained. The 
      cDNA clone comprises 665 base pairs with an open reading frame which encodes for 
      148 amino acid protein. Spleen cells expressed a significant amount of MCP-1 mRNA 
      without stimulus. But higher expression was observed when cells were stimulated 
      with Con A. MCP-1 mRNA was also expressed in tumor cell lines, although the 
      amount of expressed MCP-1 mRNA was different among cell lines.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Immunopathology Section, National Cancer Institute-Frederick Cancer Research and 
      Development Center, MD 21702.
FAU - Takeya, M
AU  - Takeya M
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
SI  - GENBANK/M57441
SI  - GENBANK/M62475
SI  - GENBANK/M62476
SI  - GENBANK/M62477
SI  - GENBANK/M62478
SI  - GENBANK/M62479
SI  - GENBANK/M62480
SI  - GENBANK/M62481
SI  - GENBANK/M62482
SI  - GENBANK/M63428
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 11028-71-0 (Concanavalin A)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*genetics/pharmacology/physiology
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Cloning, Molecular
MH  - Concanavalin A/pharmacology
MH  - DNA/genetics/isolation & purification
MH  - Gene Expression
MH  - Gene Library
MH  - Humans
MH  - Molecular Sequence Data
MH  - Poly A/genetics/isolation & purification
MH  - RNA/genetics/isolation & purification
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Inbred F344
MH  - Recombinant Proteins/pharmacology
MH  - Sequence Homology, Nucleic Acid
MH  - Spleen/drug effects/*metabolism
EDAT- 1991/01/31 00:00
MHDA- 1991/01/31 00:01
CRDT- 1991/01/31 00:00
PHST- 1991/01/31 00:00 [pubmed]
PHST- 1991/01/31 00:01 [medline]
PHST- 1991/01/31 00:00 [entrez]
AID - 0006-291X(91)91445-I [pii]
AID - 10.1016/0006-291x(91)91445-i [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1991 Jan 31;174(2):504-9. doi: 
      10.1016/0006-291x(91)91445-i.