PMID- 17043810
OWN - NLM
STAT- MEDLINE
DCOM- 20070720
LR  - 20181113
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 449
IP  - 6
DP  - 2006 Dec
TI  - Sarcoid-like lesions associated with the immune restoration inflammatory syndrome 
      in AIDS: absence of polymerase chain reaction detection of Mycobacterium 
      tuberculosis in granulomas isolated by laser capture microdissection.
PG  - 689-96
AB  - Highly active antiretroviral therapy (HAART)-treated human immunodeficiency virus 
      (HIV)-positive patients can develop granulomatous lesions within the first few 
      weeks of initiating therapy. This immune syndrome, called immune restoration 
      inflammatory syndrome (IRIS), can induce sarcoid-like lesions in tissues. The 
      pathogenesis of these granulomas is currently unknown because no pathogen has 
      been identified to date in the lesions using morphological and/or microbiological 
      approaches. However, the role of certain microbes, such as Mycobacterium 
      tuberculosis, is still debated. The aim of this study was to look for the 
      presence of M. tuberculosis in sarcoid-like lesions occurring in 14 AIDS patients 
      treated with HAART. We used the PCR DNA amplification method in granulomas 
      microdissected from sections stained by hematoxylin-eosin from formalin-fixed, 
      paraffin-embedded specimens. Results were compared to those obtained from 
      microdissected tuberculosis (TB) granulomas (15 patients) and from microdissected 
      sarcoidosis granulomas (12 patients). M. tuberculosis DNA was undetectable from 
      the microdissected sarcoid-like granulomas, whereas DNA from M. tuberculosis was 
      isolated in all the microdissected TB granulomas and was absent in the 
      microdissected sarcoidosis granulomas. Taken together, these data showed that M. 
      tuberculosis DNA is not associated with the presence of sarcoid-like lesions 
      occurring in HIV-positive patients treated with HAART.
FAU - Lassalle, Sandra
AU  - Lassalle S
AD  - INSERM ERI-21, IFR 50, University of Nice, 06107 Nice, France.
FAU - Selva, Eric
AU  - Selva E
FAU - Hofman, Veronique
AU  - Hofman V
FAU - Butori, Catherine
AU  - Butori C
FAU - Venissac, Nicolas
AU  - Venissac N
FAU - Mouroux, Jerome
AU  - Mouroux J
FAU - Dellamonica, Pierre
AU  - Dellamonica P
FAU - Hofman, Paul
AU  - Hofman P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061017
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/drug therapy/*pathology
MH  - Adult
MH  - Antiretroviral Therapy, Highly Active
MH  - DNA, Bacterial/analysis
MH  - Female
MH  - Granuloma/microbiology/*pathology
MH  - Humans
MH  - Male
MH  - Microdissection
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/*isolation & purification
MH  - *Polymerase Chain Reaction
MH  - Sarcoidosis/*pathology
EDAT- 2006/10/18 09:00
MHDA- 2007/07/21 09:00
CRDT- 2006/10/18 09:00
PHST- 2006/03/14 00:00 [received]
PHST- 2006/07/18 00:00 [accepted]
PHST- 2006/10/18 09:00 [pubmed]
PHST- 2007/07/21 09:00 [medline]
PHST- 2006/10/18 09:00 [entrez]
AID - 10.1007/s00428-006-0278-2 [doi]
PST - ppublish
SO  - Virchows Arch. 2006 Dec;449(6):689-96. doi: 10.1007/s00428-006-0278-2. Epub 2006 
      Oct 17.