PMID- 17044036
OWN - NLM
STAT- MEDLINE
DCOM- 20070313
LR  - 20181201
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 84
IP  - 8
DP  - 2006 Dec
TI  - Estrogen produced in cultured hippocampal neurons is a functional regulator of a 
      GABAergic machinery.
PG  - 1771-7
AB  - Accumulating evidence suggests that estrogen is produced locally by the neurons 
      in the brain. We observed that a 48-hr treatment with the estrogen receptor 
      antagonists ICI 182780 and tamoxifen decreased the level of glutamate 
      decarboxylase (GAD)-65, a rate-limiting gamma-aminobutyric acid 
      (GABA)-synthesizing enzyme, in a dissociated hippocampal neuronal culture. 
      Aromatase is an essential enzyme for estrogen biosynthesis. Treatment with an 
      aromatase inhibitor decreased the GAD 65 level, indicating that estrogen 
      biogenesis functions to maintain the level of this enzyme for GABAergic 
      neurotransmission. Furthermore, insofar as the effect of ICI 182780 was observed 
      equivalently in the presence of either brain-derived neurotrophic factor (BDNF) 
      or BDNF-receptor inhibitor K252a, estrogen probably regulates GAD level 
      independently of brain-derived neurotrophic factor (BDNF). Thus, estrogen 
      produced by neurons is considered to be an intrinsic regulatory factor for 
      neuronal networks that maintain GABAergic neurotransmission.
FAU - Ikeda, Takamitsu
AU  - Ikeda T
AD  - Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, 
      University of Tokyo, Tokyo, Japan.
FAU - Matsuki, Norio
AU  - Matsuki N
FAU - Yamada, Maki K
AU  - Yamada MK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Estrogens)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 22X328QOC4 (Fulvestrant)
RN  - 4TI98Z838E (Estradiol)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 2)
SB  - IM
MH  - Animals
MH  - Aromatase/pharmacology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Embryo, Mammalian
MH  - Estradiol/analogs & derivatives/pharmacology
MH  - Estrogen Antagonists/pharmacology
MH  - Estrogens/*metabolism
MH  - Fulvestrant
MH  - Gene Expression/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Glutamate Decarboxylase/metabolism
MH  - Hippocampus/*cytology
MH  - Immunohistochemistry/methods
MH  - Isoenzymes/metabolism
MH  - Neurons/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Tamoxifen/pharmacology
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 2006/10/18 09:00
MHDA- 2007/03/14 09:00
CRDT- 2006/10/18 09:00
PHST- 2006/10/18 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2006/10/18 09:00 [entrez]
AID - 10.1002/jnr.21083 [doi]
PST - ppublish
SO  - J Neurosci Res. 2006 Dec;84(8):1771-7. doi: 10.1002/jnr.21083.