PMID- 17045571
OWN - NLM
STAT- MEDLINE
DCOM- 20061218
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 350
IP  - 4
DP  - 2006 Dec 1
TI  - Estradiol regulates the insulin-like growth factor-I (IGF-I) signalling pathway: 
      a crucial role of phosphatidylinositol 3-kinase (PI 3-kinase) in estrogens 
      requirement for growth of MCF-7 human breast carcinoma cells.
PG  - 916-21
AB  - Estrogens can stimulate the proliferation of estrogen-responsive breast cancer 
      cells by increasing their proliferative response to insulin-like growth factors. 
      With a view to investigating the molecular mechanisms implicated, we studied the 
      effect of estradiol on the expression of proteins implicated in the insulin-like 
      growth factor signalling pathway. Estradiol dose- and time-dependently increased 
      the expression of insulin receptor substrate-1 and the p85/p110 subunits of 
      phosphatidylinositol 3-kinase but did not change those of ERK2 and Akt/PKB. ICI 
      182,780 did not inhibit estradiol-induced IRS-1 and p85 expression. Moreover, two 
      distinct estradiol-BSA conjugate compounds were as effective as estradiol in 
      inducing IRS-1 and p85/p110 expression indicating the possible implication of an 
      estradiol membrane receptor. Comparative analysis of steroids-depleted and 
      steroids-treated cells showed that IGF-I only stimulates cell growth in the 
      latter condition. Nevertheless, expression of a constitutively active form of PI 
      3-kinase in steroid-depleted cells triggers proliferation. These results 
      demonstrate that estradiol positively regulates essential proteins of the IGF 
      signalling pathway and put in evidence that phosphatidylinositol 3-kinase plays a 
      central role in the synergistic pro-proliferative action of estradiol and IGF-I.
FAU - Bernard, Laurence
AU  - Bernard L
AD  - Institut National de la Sante et de la Recherche Medicale, Unite 515, Croissance, 
      Differenciation et Processus Tumoraux, Hopital Saint-Antoine, Paris, France.
FAU - Legay, Christine
AU  - Legay C
FAU - Adriaenssens, Eric
AU  - Adriaenssens E
FAU - Mougel, Alexandra
AU  - Mougel A
FAU - Ricort, Jean-Marc
AU  - Ricort JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061002
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Estrogens)
RN  - 4TI98Z838E (Estradiol)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Breast Neoplasms/*pathology/*physiopathology
MH  - Cell Proliferation
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/*administration & dosage
MH  - Estrogens/*metabolism
MH  - Humans
MH  - Insulin-Like Growth Factor I/*metabolism
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Signal Transduction/*drug effects
EDAT- 2006/10/19 09:00
MHDA- 2006/12/19 09:00
CRDT- 2006/10/19 09:00
PHST- 2006/09/21 00:00 [received]
PHST- 2006/09/22 00:00 [accepted]
PHST- 2006/10/19 09:00 [pubmed]
PHST- 2006/12/19 09:00 [medline]
PHST- 2006/10/19 09:00 [entrez]
AID - S0006-291X(06)02168-1 [pii]
AID - 10.1016/j.bbrc.2006.09.116 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Dec 1;350(4):916-21. doi: 
      10.1016/j.bbrc.2006.09.116. Epub 2006 Oct 2.