PMID- 17048485
OWN - NLM
STAT- MEDLINE
DCOM- 20061208
LR  - 20190715
IS  - 1533-4880 (Print)
IS  - 1533-4880 (Linking)
VI  - 6
IP  - 9-10
DP  - 2006 Sep-Oct
TI  - Receptor-mediated gene delivery into antigen presenting cells using mannosylated 
      chitosan/DNA nanoparticles.
PG  - 2796-803
AB  - Dendritic cells (DCs) are professional antigen presenting cells that induce, 
      sustain, and regulate immune responses. Gene modification of DCs is of particular 
      interest for immunotherapy of diseases where the immunes system has failed or is 
      abnormally regulated, such as in cancer or autoimmune disease. Gene transfer 
      using non-viral vectors is a promising approach for the safe delivery of 
      therapeutic DNA. Among various non-viral vectors, chitosan is considered to be a 
      good candidate for gene delivery system, however, lack of cell specificity and 
      low transfection of chitosan need to be overcome prior to clinical use. In this 
      study, mannosylated chitosan (MC) was prepared to induce the receptor-mediated 
      endocytosis and targeting into antigen presenting cells (APCs), especially DCs 
      having mannose receptors. MC showed great ability to form complexes with DNA and 
      showed suitable physicochemical properties for gene delivery system. It had low 
      cytotoxicity and exhibited much enhanced gene transfer efficiency on the 
      macrophage cell line than chitosan itself. Also, MC/DNA complex was more 
      efficient for transferring IL-12 gene into DCs rather than water-soluble chitosan 
      (WSC)/DNA one, which resulted in better induction of INF-gamma from DCs. 
      Therefore, MC is a promising gene delivery system for repeated administration to 
      maintain sustained gene expression, thereby opening the possibility for 
      immunotherapy.
FAU - Kim, Tae Hee
AU  - Kim TH
AD  - School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, 
      Korea.
FAU - Nah, Jae Woon
AU  - Nah JW
FAU - Cho, Myung-Haing
AU  - Cho MH
FAU - Park, Tae Gwan
AU  - Park TG
FAU - Cho, Chong Su
AU  - Cho CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nanosci Nanotechnol
JT  - Journal of nanoscience and nanotechnology
JID - 101088195
RN  - 0 (Receptors, Cell Surface)
RN  - 9007-49-2 (DNA)
RN  - 9012-76-4 (Chitosan)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*metabolism
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - DNA/*administration & dosage/chemistry/*pharmacokinetics
MH  - Male
MH  - Mannose/chemistry
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Particle Size
MH  - Receptors, Cell Surface/*metabolism
MH  - Transfection/*methods
EDAT- 2006/10/20 09:00
MHDA- 2006/12/12 09:00
CRDT- 2006/10/20 09:00
PHST- 2006/10/20 09:00 [pubmed]
PHST- 2006/12/12 09:00 [medline]
PHST- 2006/10/20 09:00 [entrez]
AID - 10.1166/jnn.2006.434 [doi]
PST - ppublish
SO  - J Nanosci Nanotechnol. 2006 Sep-Oct;6(9-10):2796-803. doi: 10.1166/jnn.2006.434.