PMID- 1704932
OWN - NLM
STAT- MEDLINE
DCOM- 19910328
LR  - 20151119
IS  - 0485-1439 (Print)
IS  - 0485-1439 (Linking)
VI  - 31
IP  - 11
DP  - 1990 Nov
TI  - [Detection of antigenic determinants of autoantibodies in idiopathic 
      thrombocytopenic purpura using anti-platelet monoclonal antibodies and flow 
      cytometry in whole blood].
PG  - 1769-73
AB  - To detect antigenic sites of platelet-bound autoantibodies in chronic ITP, whole 
      blood or platelet-rich plasma from patients was stained with monoclonal 
      antibodies (MoAbs) directed against platelet membrane glycoproteins (GPs) and 
      with FITC-conjugated anti-mice IgG in an unwashed system followed by flow 
      cytometric analysis. Platelets were identified by light-scattering profile and 
      the amount of anti GP MoAb bound to platelets was measured and expressed as mean 
      fluorescence intensity. This system enabled to quantitate the amount of intact 
      GPs on platelet surface, regardless of activation of platelet. As a result, 
      decreased amount of platelet-bound anti GPIIb/IIIa MoAb was noted in 5 of 22 
      patients with chronic ITP comparing to those with normal subjects. On the 
      contrary, none of these patients revealed any demonstrable decrease in the amount 
      of anti GPIb MoAb binding. From these observations, we suggest that the decreased 
      MoAb binding to platelets in these five patients indicates the binding of 
      autoantibody directed toward antigenic determinants, which are on or close to the 
      epitope of this MoAb.
FAU - Fujisawa, K
AU  - Fujisawa K
AD  - Department of Pediatrics, Tokyo Jikei University School of Medicine.
FAU - Ikegami, M
AU  - Ikegami M
FAU - Akatsuka, J
AU  - Akatsuka J
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Rinsho Ketsueki
JT  - [Rinsho ketsueki] The Japanese journal of clinical hematology
JID - 2984782R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Epitopes)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Autoantibodies/*immunology
MH  - Blood Platelets/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Epitopes/*analysis
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - Male
MH  - Platelet Membrane Glycoproteins/analysis
MH  - Purpura, Thrombocytopenic/*diagnosis
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
PST - ppublish
SO  - Rinsho Ketsueki. 1990 Nov;31(11):1769-73.