PMID- 17050688
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 103
IP  - 44
DP  - 2006 Oct 31
TI  - Identification of conserved lipid/detergent-binding sites in a high-resolution 
      structure of the membrane protein cytochrome c oxidase.
PG  - 16117-22
AB  - Well ordered reproducible crystals of cytochrome c oxidase (CcO) from Rhodobacter 
      sphaeroides yield a previously unreported structure at 2.0 A resolution that 
      contains the two catalytic subunits and a number of alkyl chains of lipids and 
      detergents. Comparison with crystal structures of other bacterial and mammalian 
      CcOs reveals that the positions occupied by native membrane lipids and detergent 
      substitutes are highly conserved, along with amino acid residues in their 
      vicinity, suggesting a more prevalent and specific role of lipid in membrane 
      protein structure than often envisioned. Well defined detergent head groups 
      (maltose) are found associated with aromatic residues in a manner similar to 
      phospholipid head groups, likely contributing to the success of alkyl glycoside 
      detergents in supporting membrane protein activity and crystallizability. Other 
      significant features of this structure include the following: finding of a 
      previously unreported crystal contact mediated by cadmium and an engineered 
      histidine tag; documentation of the unique His-Tyr covalent linkage close to the 
      active site; remarkable conservation of a chain of waters in one proton pathway 
      (D-path); and discovery of an inhibitory cadmium-binding site at the entrance to 
      another proton path (K-path). These observations provide important insight into 
      CcO structure and mechanism, as well as the significance of bound lipid in 
      membrane proteins.
FAU - Qin, Ling
AU  - Qin L
AD  - Department of Biochemistry and Molecular Biology, Michigan State University, East 
      Lansing, MI 48824, USA.
FAU - Hiser, Carrie
AU  - Hiser C
FAU - Mulichak, Anne
AU  - Mulichak A
FAU - Garavito, R Michael
AU  - Garavito RM
FAU - Ferguson-Miller, Shelagh
AU  - Ferguson-Miller S
LA  - eng
SI  - PDB/2GSM
GR  - R01 GM026916/GM/NIGMS NIH HHS/United States
GR  - R37 GM026916/GM/NIGMS NIH HHS/United States
GR  - GM 26916/GM/NIGMS NIH HHS/United States
GR  - P01 GM 57323/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061018
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Detergents)
RN  - 0 (Lipids)
RN  - 0 (Protein Subunits)
RN  - 0 (Protons)
RN  - 00BH33GNGH (Cadmium)
RN  - 059QF0KO0R (Water)
RN  - 42HK56048U (Tyrosine)
RN  - 4QD397987E (Histidine)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Binding Sites
MH  - Cadmium/chemistry/metabolism
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Detergents/*chemistry/*metabolism
MH  - Electron Transport Complex IV/*chemistry/genetics/isolation & 
      purification/*metabolism
MH  - Electrons
MH  - Histidine/chemistry/metabolism
MH  - *Lipid Metabolism
MH  - Lipids/*chemistry
MH  - Models, Molecular
MH  - Protein Engineering
MH  - Protein Structure, Quaternary
MH  - Protein Structure, Tertiary
MH  - Protein Subunits/chemistry/genetics/metabolism
MH  - Protons
MH  - Rhodobacter sphaeroides/enzymology/genetics
MH  - Tyrosine/chemistry/metabolism
MH  - Water/chemistry
PMC - PMC1616942
COIS- The authors declare no conflict of interest.
EDAT- 2006/10/20 09:00
MHDA- 2006/12/23 09:00
PMCR- 2006/10/31
CRDT- 2006/10/20 09:00
PHST- 2006/10/20 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2006/10/20 09:00 [entrez]
PHST- 2006/10/31 00:00 [pmc-release]
AID - 0606149103 [pii]
AID - 3863 [pii]
AID - 10.1073/pnas.0606149103 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16117-22. doi: 
      10.1073/pnas.0606149103. Epub 2006 Oct 18.