PMID- 17051637
OWN - NLM
STAT- MEDLINE
DCOM- 20070226
LR  - 20071203
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 6
IP  - 22
DP  - 2006 Nov
TI  - Proteomic analysis of the ventromedial nucleus of the hypothalamus (pars 
      lateralis) in the female rat.
PG  - 6066-74
AB  - The use of proteomics to study changes in the expression of CNS proteins, which 
      may underlie the regulation of physiological and/or behavioral responses, 
      represents an emerging application of this technology. In the current study, the 
      Palkovits' microdissection method was evaluated as a means of obtaining proteomic 
      data from discrete brain nuclei. The pars lateralis of the ventromedial nucleus 
      of the hypothalamus (VMN) was chosen for the initial studies because of its 
      established role in the expression of gonadal hormone dependent female sexual 
      behavior. The VMN from ovariectomized rats was microdissected from 300 microm 
      frozen brain sections using a 500 microm punch. Total proteins were separated 
      using 2-DE. A group consensus of 432 protein spots, visualized by SYPRO Ruby 
      stain, was obtained from gels from four independent VMN samples. A low mean CV 
      and high gel-to-gel correlation coefficients indicate that reproducible 2-DE gels 
      can be generated from microdissected tissue samples. Proteins from the mediobasal 
      hypothalamus (MBH) were also separated on 2-DE gels. Evaluation of the 2-DE maps 
      from the VMN and the MBH revealed different protein profiles, and indicates that 
      microdissection improves the detection of low-abundance proteins, and reduces the 
      relative occurrence of abundant proteins on 2-DE maps.
FAU - Mo, Bing
AU  - Mo B
AD  - Department of Biology and Neuroscience Group, University of South Dakota, 
      Vermillion, SD 57069, USA.
FAU - Callegari, Eduardo
AU  - Callegari E
FAU - Telefont, Martin
AU  - Telefont M
FAU - Renner, Kenneth J
AU  - Renner KJ
LA  - eng
GR  - 2 P20 RR016479/RR/NCRR NIH HHS/United States
GR  - R01 DA019921-01/DA/NIDA NIH HHS/United States
GR  - RR15567P20/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Polyenes)
RN  - 1403-95-8 (mediocidin)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Gel, Two-Dimensional/methods
MH  - Female
MH  - Mass Spectrometry/methods
MH  - Polyenes/metabolism
MH  - Proteomics/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ventromedial Hypothalamic Nucleus/*metabolism
EDAT- 2006/10/20 09:00
MHDA- 2007/02/27 09:00
CRDT- 2006/10/20 09:00
PHST- 2006/10/20 09:00 [pubmed]
PHST- 2007/02/27 09:00 [medline]
PHST- 2006/10/20 09:00 [entrez]
AID - 10.1002/pmic.200600072 [doi]
PST - ppublish
SO  - Proteomics. 2006 Nov;6(22):6066-74. doi: 10.1002/pmic.200600072.