PMID- 17052406
OWN - NLM
STAT- MEDLINE
DCOM- 20061204
LR  - 20131121
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 8
IP  - 5
DP  - 2006 Oct
TI  - [Neurogenesis of hippocampus following pentylenetrazol-induced status epilepticus 
      in developing rats and the effect of MK-801 on neurogenesis].
PG  - 421-4
AB  - OBJECTIVE: This study aimed to determine whether pentylenetetrazol-induced status 
      epilepticus (SE) can induce dentate granule cell neurogenesis in the developing 
      rat and the effect of MK-801, a noncompetitive antagonism of N-methyl-D-aspartate 
      receptor (NMDAR), on neurogenesis. METHODS: Two hundred and sixteen postnatal 
      days 7, 14, 21 or 28 Sprague Dawley (SD) rats were involved in this study. Each 
      age group consisted of 54 rats which were randomly assigned into a SE group, a SE 
      + MK-801 group and a Normal control group (n=18 each). SE was induced by 
      intraperitoneal injection of PTZ (80 mg/kg). The SE + MK-801 group was injected 
      intraperitoneally with MK-801 (1 mg/kg) at 1 hr after SE episode. All rats were 
      given 5-bromodeoxyuridene (BrdU) intraperitonealy to label newborn cells at 6, 13 
      and 27 days after seizures and then were sacrificed 24 hrs after BrdU injection. 
      The immunohistochemistry method was used to measure the expression of BrdU, TuJl 
      (betaIII tubulin), and glial fibrillary acidic protein (GFAP) in the dentate 
      gyrus of hippocampus of rats. RESULTS: The number of the BrdU positive cells in 
      the SE group was significantly higher than in the age-matched normal controls at 
      7 and 14 days after SE episode (P <0.05 or 0.01). Approximately 82.5% and 80.3% 
      of BrdU-labeled cells in the SE and the Control groups were co-expressed TuJ1 
      respectively. MK-801 treatment decreased the BrdU positive cells compared with 
      the SE group at 7 and 14 days after SE seizures (P < 0.01). On the 28th day after 
      SE episode there were no differences among the three groups for the BrdU positive 
      cells. CONCLUSIONS: PTZ-induced SE can increase the dentate granule cell 
      neurogenesis in the developing rat. NMDAR plays an important role in neurogenesis 
      following seizures.
FAU - Chen, Jing
AU  - Chen J
AD  - Department of Pediatrics, Affiliated Hospital of Xuzhou College, Xuzhou, Jiangsu 
      221002, China.
FAU - Yuan, Bao-Qiang
AU  - Yuan BQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Dentate Gyrus/drug effects/*physiopathology
MH  - Dizocilpine Maleate/*pharmacology
MH  - Female
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Immunohistochemistry
MH  - Male
MH  - Pentylenetetrazole
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Status Epilepticus/chemically induced/*physiopathology
EDAT- 2006/10/21 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/10/21 09:00
PHST- 2006/10/21 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/21 09:00 [entrez]
AID - 1008-8830(2006)05-0421-04 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2006 Oct;8(5):421-4.