PMID- 17052738
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20091119
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 359
IP  - 1
DP  - 2007 Mar 1
TI  - T cell line passage can select for pre-existing neutralization-sensitive variants 
      from the quasispecies of primary human immunodeficiency virus type-1 isolates.
PG  - 92-104
AB  - Primary human immunodeficiency type 1 viruses (HIV-1) resist antibody 
      neutralization but become sensitive after passage through T cell lines. We and 
      others previously reported an increased neutralization sensitivity of HIV-1 after 
      prolonged culture on primary peripheral blood mononuclear cells (PBMC). Hence we 
      hypothesized that adaptation to growth in T cell lines is in fact selection of a 
      pre-existing neutralization-sensitive HIV-1 variant from the quasispecies in the 
      PBMC culture. Indeed, increased neutralization sensitivity was associated with 
      largely identical synonymous and non-synonymous mutations between progeny of 
      parallel H9 passages from the same split inoculum from 2 of 3 viruses. H9 T cell 
      line adaptation of molecular cloned HIV-1 was less successful and associated with 
      only a few de novo mutations that varied between parallel H9-adapted progeny from 
      the same split inoculum. We conclude that T cell line adaptation of HIV-1 can 
      indeed select for a pre-existing variant but that this most likely depends on the 
      viral diversity in the inoculum.
FAU - Quakkelaar, Esther D
AU  - Quakkelaar ED
AD  - Department of Clinical Viro-Immunology, Sanquin Research and Landsteiner 
      Laboratory of the Academic Medical Center, Center for Infection and Immunity 
      Amsterdam, University of Amsterdam, Amsterdam, The Netherlands. 
      e.quakkelaar@sanquin.nl
FAU - Beaumont, Tim
AU  - Beaumont T
FAU - van Nuenen, Ad C
AU  - van Nuenen AC
FAU - van Alphen, Floris P J
AU  - van Alphen FP
FAU - Boeser-Nunnink, Brigitte D M
AU  - Boeser-Nunnink BD
FAU - van 't Wout, Angelique B
AU  - van 't Wout AB
FAU - Schuitemaker, Hanneke
AU  - Schuitemaker H
LA  - eng
SI  - GENBANK/EF028243
SI  - GENBANK/EF028244
SI  - GENBANK/EF028245
SI  - GENBANK/EF028246
SI  - GENBANK/EF028247
SI  - GENBANK/EF028248
SI  - GENBANK/EF028249
SI  - GENBANK/EF028250
SI  - GENBANK/EF028251
SI  - GENBANK/EF028252
SI  - GENBANK/EF028253
SI  - GENBANK/EF028254
SI  - GENBANK/EF028255
SI  - GENBANK/EF028256
SI  - GENBANK/EF028257
SI  - GENBANK/EF028258
SI  - GENBANK/EF028259
SI  - GENBANK/EF028260
SI  - GENBANK/EF028261
SI  - GENBANK/EF028262
SI  - GENBANK/EF028263
SI  - GENBANK/EF028264
SI  - GENBANK/EF028265
SI  - GENBANK/EF028266
SI  - GENBANK/EF028267
SI  - GENBANK/EF028268
SI  - GENBANK/EF028269
SI  - GENBANK/EF028270
SI  - GENBANK/EF028271
SI  - GENBANK/EF028272
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061018
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (HIV Antibodies)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adaptation, Physiological
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Line
MH  - Genetic Variation
MH  - HIV Antibodies/*immunology
MH  - HIV-1/*genetics/growth & development/*immunology/isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neutralization Tests
MH  - Phylogeny
MH  - Proviruses/genetics
MH  - RNA, Viral/genetics
MH  - *Selection, Genetic
MH  - Sequence Alignment
MH  - T-Lymphocytes/*virology
EDAT- 2006/10/21 09:00
MHDA- 2007/04/04 09:00
CRDT- 2006/10/21 09:00
PHST- 2006/06/29 00:00 [received]
PHST- 2006/08/01 00:00 [revised]
PHST- 2006/09/18 00:00 [accepted]
PHST- 2006/10/21 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2006/10/21 09:00 [entrez]
AID - S0042-6822(06)00676-3 [pii]
AID - 10.1016/j.virol.2006.09.021 [doi]
PST - ppublish
SO  - Virology. 2007 Mar 1;359(1):92-104. doi: 10.1016/j.virol.2006.09.021. Epub 2006 
      Oct 18.