PMID- 17052849 OWN - NLM STAT- MEDLINE DCOM- 20070123 LR - 20131121 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 410 IP - 1 DP - 2006 Dec 13 TI - Modulation of BDNF and TrkB expression in rat hippocampus in response to acute neurotoxicity by diethyldithiocarbamate. PG - 66-70 AB - In this study, we examined the expression profile of brain-derived neurotrophic factor (BDNF) and its receptor TrkB in adult rat hippocampus following acute administration of diethyldithiocarbamate (DDTC), a neurotoxic compound which was previously shown to induce microglia activation and cell death. Semiquantitative RT-PCR analysis detected significant variations of BDNF mRNA levels in whole hippocampus homogenates, with a peak at 24h after DDTC injection. Increased BDNF protein expression was demonstrated by immunohistochemistry in various hippocampal subfields. The most relevant increase was observed in the hilus of the dentate gyrus where BDNF levels at 120h were found to be almost four times those of basal levels. Full-length TrkB (TrkB.FL) encoding mRNA was also shown to undergo an earlier increase in the hippocampus of DDTC-treated rats. TrkB immunostaining with an antibody binding both full-length and truncated (TrkB.T) isoforms was found to increase at 120h in the hippocampal CA2 and CA3 regions. These results demonstrate that DDTC modulates the expression of BDNF and its receptor in the adult rat hippocampus and suggest a possible involvement of this neurotrophin in the protective response to DDTC-induced neuronal damage. FAU - Micheli, M R AU - Micheli MR AD - Dipartimento di Biologia Cellulare e Ambientale, Universita di Perugia, Via Pascoli, 06123 Perugia, Italy. marita@unipg.it FAU - Bova, R AU - Bova R FAU - Laurenzi, M A AU - Laurenzi MA FAU - Bazzucchi, M AU - Bazzucchi M FAU - Grassi Zucconi, G AU - Grassi Zucconi G LA - eng PT - Comparative Study PT - Journal Article DEP - 20061018 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neurotoxins) RN - 0 (RNA, Messenger) RN - 99Z2744345 (Ditiocarb) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Ditiocarb/*pharmacology MH - Gene Expression/drug effects MH - Gene Expression Regulation/*drug effects MH - Hippocampus/*drug effects/metabolism MH - Immunohistochemistry/methods MH - Male MH - Neurotoxins/*pharmacology MH - RNA, Messenger/metabolism MH - Rats MH - Rats, Wistar MH - Receptor, trkB/genetics/*metabolism MH - Reverse Transcriptase Polymerase Chain Reaction/methods MH - Time Factors EDAT- 2006/10/21 09:00 MHDA- 2007/01/24 09:00 CRDT- 2006/10/21 09:00 PHST- 2006/06/28 00:00 [received] PHST- 2006/09/26 00:00 [revised] PHST- 2006/09/27 00:00 [accepted] PHST- 2006/10/21 09:00 [pubmed] PHST- 2007/01/24 09:00 [medline] PHST- 2006/10/21 09:00 [entrez] AID - S0304-3940(06)01045-7 [pii] AID - 10.1016/j.neulet.2006.09.069 [doi] PST - ppublish SO - Neurosci Lett. 2006 Dec 13;410(1):66-70. doi: 10.1016/j.neulet.2006.09.069. Epub 2006 Oct 18.