PMID- 1705320
OWN - NLM
STAT- MEDLINE
DCOM- 19910402
LR  - 20071114
IS  - 0890-6467 (Print)
IS  - 0890-6467 (Linking)
VI  - 6
IP  - 1
DP  - 1991
TI  - Characterization of Int-5, a locus associated with early events in mammary 
      carcinogenesis.
PG  - 53-63
AB  - Three chemically-induced precancerous mammary hyperplasias, independently 
      isolated in BALB/c mice, all contained mouse mammary tumor virus (MMTV) proviral 
      DNA integrated into a common region in chromosomal DNA, designated Int-5 
      (Formerly Int-H, Gray et al., 1986). This site was cloned from a hyperplastic 
      outgrowth (D2) into lambda phage. A 1.7 Kb Hind III DNA fragment, which flanks 
      the 5' end of the MMTV insert, was generated from the cloned Int-5 region. This 
      fragment was used as probe (IH-2) to localize Int-5 to mouse chromosome 9. The 
      IH-2 sequence was highly conserved in DNA of several mammalian species including 
      man, in three other widely divergent vertebrate phyla, and in C. elegans. The 
      Int-5 region, containing 5.6 Kb 5' and 12.8 Kb 3' to the MMTV integration site in 
      D2, was cloned in EMBL-3 from a BALB/c genomic DNA library. cDNA complementary to 
      poly A+ lactating mammary gland RNA, annealed with Sst-1 fragments spanning most 
      of the BALB/c Int-5 clone. The highest level of Int-5 specific poly A+ mRNA was 
      detected in D2 tumor. Lactating mammary gland and D2 hyperplastic alveolar nodule 
      contained 5-fold less Int-5 RNA while liver contained 8-fold less Int-5 RNA. 
      Int-5 cDNA (IH-3) annealed with two RNA species of approximately 3.3 and 4.0 Kb. 
      These data are consistent with the hypothesis that Int-5 contains an oncogene, 
      different from any other previously described, involved in early events in some 
      models of chemical carcinogenesis.
FAU - Morris, V L
AU  - Morris VL
AD  - Department of Microbiology and Immunology, University of Western Ontario, London, 
      Canada.
FAU - Rao, T R
AU  - Rao TR
FAU - Kozak, C A
AU  - Kozak CA
FAU - Gray, D A
AU  - Gray DA
FAU - Lee Chan, E C
AU  - Lee Chan EC
FAU - Cornell, T J
AU  - Cornell TJ
FAU - Taylor, C B
AU  - Taylor CB
FAU - Jones, R F
AU  - Jones RF
FAU - McGrath, C M
AU  - McGrath CM
LA  - eng
GR  - CA44901/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Oncogene Res
JT  - Oncogene research
JID - 8801457
RN  - 0 (DNA Probes)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Cloning, Molecular
MH  - DNA/analysis
MH  - DNA Probes
MH  - Mammary Neoplasms, Experimental/*genetics/microbiology
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nucleic Acid Hybridization
MH  - RNA/analysis
MH  - Restriction Mapping
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene Res. 1991;6(1):53-63.