PMID- 17053314
OWN - NLM
STAT- MEDLINE
DCOM- 20070118
LR  - 20190823
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 50
IP  - 10
DP  - 2006
TI  - Detection of enteroviruses and mammalian reoviruses in Korean environmental 
      waters.
PG  - 781-6
AB  - Using the standard total culturable virus assay-most probable number (TCVA-MPN) 
      method, we evaluated a total of 348 samples, including surface water, finished 
      water, and tap water samples, collected from randomly selected water treatment 
      plants in Korea from August 2001 through July 2005 according to the Information 
      Collection Rule. All the TCVA-positive samples were also subjected to integrated 
      cell culture-PCR (ICC-PCR) methods for the detection of enteroviruses, hepatitis 
      A virus, adenoviruses, and reoviruses. The most probable number of infectious 
      units per 100 liters for the environmental water samples ranged from 0.5 to 47.3. 
      Nine of the 13 TCVA-positive samples (69.2%) were found by ICC-PCR to be positive 
      for human enteroviruses, which were confirmed to be coxsackievirus type B3, 
      coxsackievirus type B4, coxsackievirus type B6, echovirus type 30, and vaccine 
      strain poliovirus type 3 by direct sequencing. Eleven of the 13 TCVA-positive 
      samples (84.6%) were found by ICC-PCR assay to be positive for reoviruses. The 
      serotype of all the reoviruses was the same as reovirus type 1 by direct 
      sequencing. Both enteroviruses and reoviruses were concurrently detected in seven 
      TCVA-positive samples (53.8%).
FAU - Kim, Hee Jung
AU  - Kim HJ
AD  - Department of Microbiology, College of Medicine, Kangwon University, Hyoja 
      2-Dong, Chunchon, Kangwon-Do 200-701, Korea.
FAU - Shin, Young Oh
AU  - Shin YO
FAU - Kim, Sang Hyun
AU  - Kim SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
SB  - IM
MH  - Enterovirus/*isolation & purification
MH  - Polymerase Chain Reaction
MH  - Reoviridae/*isolation & purification
MH  - *Water Microbiology
MH  - Water Supply
EDAT- 2006/10/21 09:00
MHDA- 2007/01/19 09:00
CRDT- 2006/10/21 09:00
PHST- 2006/10/21 09:00 [pubmed]
PHST- 2007/01/19 09:00 [medline]
PHST- 2006/10/21 09:00 [entrez]
AID - JST.JSTAGE/mandi/50.781 [pii]
AID - 10.1111/j.1348-0421.2006.tb03854.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2006;50(10):781-6. doi: 10.1111/j.1348-0421.2006.tb03854.x.