PMID- 17055046
OWN - NLM
STAT- MEDLINE
DCOM- 20070126
LR  - 20131121
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 28
IP  - 4
DP  - 2007 Feb
TI  - Shell-crosslinked Pluronic L121 micelles as a drug delivery vehicle.
PG  - 725-34
AB  - Pluronic block copolymers (PBCs) have been shown to reverse multidrug resistance 
      (MDR) by inhibiting the P-glycoprotein (P-gp) pump in cancer cells. One of the 
      problems encountered with the use of PBCs is that the micelles disassociate at 
      low concentrations. The study focused on the stabilization of PBC L121 micelles 
      by the formation of crosslinks within their outer shells. To form crosslinks, the 
      two terminal alcohols on L121 were first chemically converted into aldehydes 
      (L121-CHO) using the Dess-Martin periodinane. Diamine compounds were then used to 
      bridge the converted aldehyde termini on L121-CHO via conjugated Schiff bases. 
      After crosslinking, the morphology of the L121 micelles remained spherical in 
      shape and the mean particle sizes of the micelles before and after crosslinking 
      were comparable (100nm). After exposure of MDR KBv cells to free rhodamine-123 
      (R123), the accumulation of R123 in cells was limited due to the function of 
      P-gp. In contrast, crosslinking of L121 micelles within their outer shells 
      significantly reduced their critical micelle concentration and greatly enhanced 
      their stability, while maintaining their ability to inhibit P-gp function in 
      resistant cells. The results indicated that the L121 micelles with shell 
      crosslinks may be useful as a drug delivery vehicle for cancer chemotherapy.
FAU - Yang, Ting-Fan
AU  - Yang TF
AD  - Department of Chemical Engineering/Bioengineering Program, National Tsing Hua 
      University, Hsinchu, Taiwan 30013, ROC.
FAU - Chen, Chun-Nan
AU  - Chen CN
FAU - Chen, Mei-Chin
AU  - Chen MC
FAU - Lai, Chien-Hsun
AU  - Lai CH
FAU - Liang, Hsiang-Fa
AU  - Liang HF
FAU - Sung, Hsing-Wen
AU  - Sung HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061019
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Drug Carriers)
RN  - 0 (Micelles)
RN  - 106392-12-5 (Poloxamer)
RN  - V10TVZ52E4 (Putrescine)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cell Survival/drug effects
MH  - Cricetinae
MH  - Cross-Linking Reagents/*chemistry
MH  - Drug Carriers/*chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - *Micelles
MH  - Microscopy, Electron, Transmission
MH  - Molecular Structure
MH  - Poloxamer/*chemistry/toxicity
MH  - Putrescine/chemistry
MH  - Temperature
EDAT- 2006/10/24 09:00
MHDA- 2007/01/27 09:00
CRDT- 2006/10/24 09:00
PHST- 2006/08/14 00:00 [received]
PHST- 2006/09/26 00:00 [accepted]
PHST- 2006/10/24 09:00 [pubmed]
PHST- 2007/01/27 09:00 [medline]
PHST- 2006/10/24 09:00 [entrez]
AID - S0142-9612(06)00845-3 [pii]
AID - 10.1016/j.biomaterials.2006.09.035 [doi]
PST - ppublish
SO  - Biomaterials. 2007 Feb;28(4):725-34. doi: 10.1016/j.biomaterials.2006.09.035. 
      Epub 2006 Oct 19.