PMID- 17056700
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20191210
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 12
DP  - 2006 Dec
TI  - Population dynamics of Bifidobacterium species in human feces during raffinose 
      administration monitored by fluorescence in situ hybridization-flow cytometry.
PG  - 7739-47
AB  - The population dynamics of bifidobacteria in human feces during raffinose 
      administration were investigated at the species level by using fluorescence in 
      situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although 
      double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and 
      indodicarbocyanine (Cy5) as labeling dyes for fecal samples has been reported, 
      the analysis was interfered with by strong autofluorescence at the FITC 
      fluorescence region because of the presence of autofluorescence particles/debris 
      in the fecal samples. We circumvented this problem by using only Cy5 fluorescent 
      dye in the FISH-FCM analysis. Thirteen subjects received 2 g of raffinose twice a 
      day for 4 weeks. Fecal samples were collected, and the bifidobacterial 
      populations were monitored using the established FISH-FCM method. The results 
      showed an increase in bifidobacteria from about 12.5% of total bacteria in the 
      prefeeding period to about 28.7 and 37.2% after the 2-week and 4-week feeding 
      periods, respectively. Bifidobacterium adolescentis, the Bifidobacterium 
      catenulatum group, and Bifidobacterium longum were the major species, in that 
      order, at the prefeeding period, and these bacteria were found to increase nearly 
      in parallel during the raffinose administration. During the feeding periods, 
      indigenous bifidobacterial populations became more diverse, such that minor 
      species in human adults, such as Bifidobacterium breve, Bifidobacterium bifidum, 
      Bifidobacterium dentium, and Bifidobacterium angulatum, proliferated. Four weeks 
      after raffinose administration was stopped, the proportion of each major 
      bifidobacterial species, as well as that of total bifidobacteria, returned to 
      approximately the original values for the prefeeding period, whereas that of each 
      minor species appeared to differ considerably from its original value. To the 
      best of our knowledge, these results provide the first clear demonstration of the 
      population dynamics of indigenous bifidobacteria at the species level in response 
      to raffinose administration.
FAU - Dinoto, Achmad
AU  - Dinoto A
AD  - Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido 
      University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
FAU - Marques, Tatiana M
AU  - Marques TM
FAU - Sakamoto, Kanta
AU  - Sakamoto K
FAU - Fukiya, Satoru
AU  - Fukiya S
FAU - Watanabe, Jun
AU  - Watanabe J
FAU - Ito, Susumu
AU  - Ito S
FAU - Yokota, Atsushi
AU  - Yokota A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061020
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - N5O3QU595M (Raffinose)
SB  - IM
MH  - Adult
MH  - Bifidobacterium/classification/drug effects/genetics/*growth & development
MH  - Feces/*microbiology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Raffinose/*administration & dosage
MH  - Species Specificity
PMC - PMC1694202
EDAT- 2006/10/24 09:00
MHDA- 2007/01/24 09:00
PMCR- 2007/04/01
CRDT- 2006/10/24 09:00
PHST- 2006/10/24 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/10/24 09:00 [entrez]
PHST- 2007/04/01 00:00 [pmc-release]
AID - AEM.01777-06 [pii]
AID - 1777-06 [pii]
AID - 10.1128/AEM.01777-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Dec;72(12):7739-47. doi: 10.1128/AEM.01777-06. Epub 
      2006 Oct 20.