PMID- 17057234
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20181113
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 175
IP  - 1
DP  - 2007 Jan
TI  - Cytological visualization of DNA transposons and their transposition pattern in 
      somatic cells of maize.
PG  - 31-9
AB  - Global genomic analysis of transposable element distributions of both natural 
      (En/Spm, Ac-Ds, and MuDR/Mu) and modified (RescueMu) types was performed by 
      fluorescence in situ hybridization (FISH) on somatic chromosomes coupled with 
      karyotyping of each chromosome. In lines without an active transposable element, 
      the locations of silent En/Spm, Ac-Ds, and MuDR/Mu elements were visualized, 
      revealing variation in copy number and position among lines but no apparent 
      locational bias. The ability to detect single elements was validated by using 
      previously mapped active Ac elements. Somatic transpositions were documented in 
      plants containing an engineered Mutator element, RescueMu, via use of the 
      karyotyping system. By analyzing the RescueMu lines, we found that transposition 
      of RescueMu in root-tip cells follows the cut-and-paste type of transposition. 
      This work demonstrates the utility of FISH and karyotyping in the study of 
      transposon activity and its consequences.
FAU - Yu, Weichang
AU  - Yu W
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211, USA.
FAU - Lamb, Jonathan C
AU  - Lamb JC
FAU - Han, Fangpu
AU  - Han F
FAU - Birchler, James A
AU  - Birchler JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20061022
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
RN  - EC 2.7.7.- (Transposases)
SB  - IM
MH  - DNA Transposable Elements/*genetics
MH  - DNA, Plant/genetics
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Plasmids
MH  - Transposases/genetics/metabolism
MH  - Zea mays/*genetics/growth & development
PMC - PMC1775000
EDAT- 2006/10/24 09:00
MHDA- 2007/04/04 09:00
PMCR- 2007/04/01
CRDT- 2006/10/24 09:00
PHST- 2006/10/24 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2006/10/24 09:00 [entrez]
PHST- 2007/04/01 00:00 [pmc-release]
AID - genetics.106.064238 [pii]
AID - gen175131 [pii]
AID - 10.1534/genetics.106.064238 [doi]
PST - ppublish
SO  - Genetics. 2007 Jan;175(1):31-9. doi: 10.1534/genetics.106.064238. Epub 2006 Oct 
      22.