PMID- 17057708
OWN - NLM
STAT- MEDLINE
DCOM- 20061219
LR  - 20220309
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 9
IP  - 11
DP  - 2006 Nov
TI  - IGF-I specifically enhances axon outgrowth of corticospinal motor neurons.
PG  - 1371-81
AB  - Corticospinal motor neurons (CSMN) are among the most complex CNS neurons; they 
      control voluntary motor function and are prototypical projection neurons. In 
      amyotrophic lateral sclerosis (ALS), both spinal motor neurons and CSMN 
      degenerate; their damage contributes centrally to the loss of motor function in 
      spinal cord injury. Direct investigation of CSMN is severely limited by 
      inaccessibility in the heterogeneous cortex. Here, using new CSMN purification 
      and culture approaches, and in vivo analyses, we report that insulin-like growth 
      factor-1 (IGF-I) specifically enhances the extent and rate of murine CSMN axon 
      outgrowth, mediated via the IGF-I receptor and downstream signaling pathways; 
      this is distinct from IGF-I support of neuronal survival. In contrast, 
      brain-derived neurotrophic factor (BDNF) enhances branching and arborization, but 
      not axon outgrowth. These experiments define specific controls over directed 
      differentiation of CSMN, indicate a distinct role of IGF-I in CSMN axon outgrowth 
      during development, and might enable control over CSMN derived from neural 
      precursors.
FAU - Ozdinler, P Hande
AU  - Ozdinler PH
AD  - MGH-HMS Center for Nervous System Repair, Department of Neurosurgery, Harvard 
      Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
FAU - Macklis, Jeffrey D
AU  - Macklis JD
LA  - eng
GR  - NS41590/NS/NINDS NIH HHS/United States
GR  - NS45523/NS/NINDS NIH HHS/United States
GR  - NS49553/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061022
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
CIN - Nat Neurosci. 2006 Nov;9(11):1357. PMID: 17066065
MH  - Animals
MH  - Axons/*physiology
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cell Separation
MH  - Cell Size
MH  - Cell Survival/physiology
MH  - Flow Cytometry
MH  - Insulin-Like Growth Factor I/*physiology
MH  - Mice
MH  - Motor Neurons/*physiology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Pyramidal Tracts/cytology/*physiology
MH  - Signal Transduction/drug effects/physiology
EDAT- 2006/10/24 09:00
MHDA- 2006/12/21 09:00
CRDT- 2006/10/24 09:00
PHST- 2006/08/21 00:00 [received]
PHST- 2006/09/25 00:00 [accepted]
PHST- 2006/10/24 09:00 [pubmed]
PHST- 2006/12/21 09:00 [medline]
PHST- 2006/10/24 09:00 [entrez]
AID - nn1789 [pii]
AID - 10.1038/nn1789 [doi]
PST - ppublish
SO  - Nat Neurosci. 2006 Nov;9(11):1371-81. doi: 10.1038/nn1789. Epub 2006 Oct 22.