PMID- 17059521
OWN - NLM
STAT- MEDLINE
DCOM- 20070507
LR  - 20220408
IS  - 0269-2813 (Print)
IS  - 0269-2813 (Linking)
VI  - 24
IP  - 9
DP  - 2006 Nov 1
TI  - Diagnostic significance of HLA-DQ typing in patients with previous coeliac 
      disease diagnosis based on histology alone.
PG  - 1395-402
AB  - BACKGROUND: Coeliac disease is strongly associated with human leukocyte antigen 
      (HLA)-DQ2 or DQ8 genotypes. The diagnosis is based on demonstrating 
      crypt-hyperplastic villous atrophy, endomysial or transglutaminase antibodies and 
      correlation of disease activity with gluten intake. AIM: To evaluate the clinical 
      utility of HLA-DQ typing, when coeliac disease diagnosis had previously been 
      established solely by histology. METHODS: HLA-DQ alleles, endomysial and 
      transglutaminase antibodies were investigated and histology slides reviewed in 70 
      patients diagnosed 2-25 years earlier by small-intestinal biopsy but without 
      measuring endomysial or transglutaminase antibodies. Patients without DQ2 or DQ8 
      or without unequivocal villous atrophy were followed-up on free diet by using 
      serology and biopsies. RESULTS: All 40 endomysial/transglutaminase antibodies 
      positive patients carried DQ2 or DQ8, and 39 of them had severe villous atrophy. 
      Only 56% of patients without endomysial or transglutaminase antibodies positivity 
      had DQ2 or DQ8 (P < 0.001). Seropositivity and relapse developed in 4 of 11 DQ2 
      positive but in none of 15 DQ2 and DQ8 negative patients on long-term gluten 
      exposure. CONCLUSIONS: Coeliac disease diagnosis based solely on histology is not 
      always reliable. HLA-DQ typing is important in identifying DQ2 and DQ8 negative 
      subjects who need revision of their diagnosis, but it does not have additive 
      diagnostic value if endomysial positivity is already known.
FAU - Kapitany, A
AU  - Kapitany A
AD  - Research Group of Autoimmune Diseases, Hungarian Academy of Sciences, Debrecen, 
      Hungary.
FAU - Toth, L
AU  - Toth L
FAU - Tumpek, J
AU  - Tumpek J
FAU - Csipo, I
AU  - Csipo I
FAU - Sipos, E
AU  - Sipos E
FAU - Woolley, N
AU  - Woolley N
FAU - Partanen, J
AU  - Partanen J
FAU - Szegedi, G
AU  - Szegedi G
FAU - Olah, E
AU  - Olah E
FAU - Sipka, S
AU  - Sipka S
FAU - Korponay-Szabo, I R
AU  - Korponay-Szabo IR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Aliment Pharmacol Ther
JT  - Alimentary pharmacology & therapeutics
JID - 8707234
RN  - 0 (HLA-DQ Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Celiac Disease/blood/*diagnosis/*genetics
MH  - Child
MH  - Child, Preschool
MH  - Genetic Predisposition to Disease
MH  - HLA-DQ Antigens/blood/*genetics
MH  - *Histocompatibility Testing
MH  - Humans
EDAT- 2006/10/25 09:00
MHDA- 2007/05/08 09:00
CRDT- 2006/10/25 09:00
PHST- 2006/10/25 09:00 [pubmed]
PHST- 2007/05/08 09:00 [medline]
PHST- 2006/10/25 09:00 [entrez]
AID - APT3133 [pii]
AID - 10.1111/j.1365-2036.2006.03133.x [doi]
PST - ppublish
SO  - Aliment Pharmacol Ther. 2006 Nov 1;24(9):1395-402. doi: 
      10.1111/j.1365-2036.2006.03133.x.