PMID- 1705990
OWN - NLM
STAT- MEDLINE
DCOM- 19910417
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 65
IP  - 4
DP  - 1991 Apr
TI  - Dengue virus-specific, human CD4+ CD8- cytotoxic T-cell clones: multiple patterns 
      of virus cross-reactivity recognized by NS3-specific T-cell clones.
PG  - 1823-8
AB  - Thirteen dengue virus-specific, cytotoxic CD4+ CD8- T-cell clones were 
      established from a donor who was infected with dengue virus type 3. These clones 
      were examined for virus specificity and human leukocyte antigen (HLA) restriction 
      in cytotoxic assays. Six patterns of virus specificities were determined. Two 
      serotype-specific clones recognized only dengue virus type 3. Two dengue virus 
      subcomplex-specific clones recognized dengue virus types 2, 3, and 4, and one 
      subcomplex-specific clone recognized dengue virus types 1, 2, and 3. Four dengue 
      virus serotype-cross-reactive clones recognized dengue virus types 1, 2, 3, and 
      4. One flavivirus-cross-reactive clone recognized dengue virus types 1, 2, 3, and 
      4 and West Nile virus (WNV), but did not recognize yellow fever virus (YFV), 
      whereas three flavivirus-cross-reactive clones recognized dengue virus types 1, 
      2, 3, and 4, WNV, and YFV. HLA restriction in the lysis by these T-cell clones 
      was also heterogeneous. HLA-DP, HLA-DQ, and HLA-DR were used as restriction 
      elements by various T-cell clones. We also examined the recognition of viral 
      nonstructural protein NS3, purified from cells infected with dengue virus type 3 
      or WNV, by these T-cell clones. One serotype-specific clone, two dengue virus 
      subcomplex-specific clones, and three dengue virus serotype-cross-reactive clones 
      recognized NS3 of dengue virus type 3. One flavivirus-cross-reactive clone 
      recognized NS3 of dengue virus type 3 and WNV. These results indicate that 
      heterogeneous dengue virus-specific CD4+ cytotoxic T cells are stimulated in 
      response to infection with a dengue virus and that a nonstructural protein, NS3, 
      contains multiple dominant T-cell epitopes.
FAU - Kurane, I
AU  - Kurane I
AD  - Department of Medicine, University of Massachusetts Medical Center, Worcester 
      01655.
FAU - Brinton, M A
AU  - Brinton MA
FAU - Samson, A L
AU  - Samson AL
FAU - Ennis, F A
AU  - Ennis FA
LA  - eng
GR  - T32-AI07272/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (NS3 protein, flavivirus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.- (RNA Nucleotidyltransferases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology/*microbiology
MH  - Cells, Cultured
MH  - Clone Cells/immunology/microbiology
MH  - Cross Reactions
MH  - Cytotoxicity, Immunologic
MH  - Dengue/*immunology
MH  - Dengue Virus/*immunology
MH  - Epitopes
MH  - HLA Antigens/immunology
MH  - Humans
MH  - RNA Helicases
MH  - RNA Nucleotidyltransferases/immunology
MH  - Sensitivity and Specificity
MH  - Serine Endopeptidases
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - *Viral Nonstructural Proteins
MH  - Viral Proteins/*immunology
PMC - PMC239991
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
PMCR- 1991/04/01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PHST- 1991/04/01 00:00 [pmc-release]
AID - 10.1128/JVI.65.4.1823-1828.1991 [doi]
PST - ppublish
SO  - J Virol. 1991 Apr;65(4):1823-8. doi: 10.1128/JVI.65.4.1823-1828.1991.