PMID- 17061253
OWN - NLM
STAT- MEDLINE
DCOM- 20070313
LR  - 20220825
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 84
IP  - 8
DP  - 2006 Dec
TI  - Modeling the neurovascular niche: VEGF- and BDNF-mediated cross-talk between 
      neural stem cells and endothelial cells: an in vitro study.
PG  - 1656-68
AB  - Neural stem cells (NSCs) exist in vascularized niches. Although there has been 
      ample evidence supporting a role for endothelial cell-derived soluble factors as 
      modulators of neural stem cell self-renewal and neuronal differentiation there is 
      a paucity of data reported on neural stem cell modulation of endothelial cell 
      behavior. We show that co-culture of NSCs with brain-derived endothelial cells 
      (BECs) either in direct contact or separated by a porous membrane elicited robust 
      vascular tube formation and maintenance, mediated by induction of vascular 
      vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor 
      (BDNF) and activation of vascular VEGFR2 and TrkB by NSC NO. Nitric oxide (NO) 
      scavengers and sequestration of VEGF and BDNF blunted this induction of tube 
      formation, whereas addition of exogenous NO donor, rBDNF and rVEGF rescued the 
      induction of tube formation. Further, rBDNF enhanced NSC eNOS activation and NO 
      generation, suggesting an inducible positive feed-back signaling loop between 
      NSCs and BECs, providing for homeostasis and responsiveness of the resident NSCs 
      and BECs comprising the neurovascular niche. These findings show the importance 
      of reciprocal modulation of NSCs and BECs in induction and maintenance of the 
      neurovascular niche and underscores their dynamic interactions.
FAU - Li, Qi
AU  - Li Q
AD  - Department of Pathology, Yale University, School of Medicine, New Haven, CT 
      06520, USA.
FAU - Ford, Millicent C
AU  - Ford MC
FAU - Lavik, Erin B
AU  - Lavik EB
FAU - Madri, Joseph A
AU  - Madri JA
LA  - eng
GR  - P01-NS35476/NS/NINDS NIH HHS/United States
GR  - R37-HL28373/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/cytology
MH  - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology
MH  - Cell Communication/*drug effects/physiology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques/methods
MH  - Endothelial Cells/*drug effects/physiology/ultrastructure
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Green Fluorescent Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Microscopy, Electron, Transmission/methods
MH  - Models, Biological
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*drug effects/physiology/ultrastructure
MH  - Nitric Oxide/metabolism
MH  - Platelet Endothelial Cell Adhesion Molecule-1/genetics
MH  - Stem Cells/*drug effects/physiology
MH  - Vascular Endothelial Growth Factor A/metabolism/*pharmacology
EDAT- 2006/10/25 09:00
MHDA- 2007/03/14 09:00
CRDT- 2006/10/25 09:00
PHST- 2006/10/25 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2006/10/25 09:00 [entrez]
AID - 10.1002/jnr.21087 [doi]
PST - ppublish
SO  - J Neurosci Res. 2006 Dec;84(8):1656-68. doi: 10.1002/jnr.21087.