PMID- 17063385
OWN - NLM
STAT- MEDLINE
DCOM- 20080303
LR  - 20181113
IS  - 1567-2379 (Print)
IS  - 1567-2379 (Linking)
VI  - 37
IP  - 3-4
DP  - 2006 May
TI  - Immunohistochemical study of monocyte chemoattractant protein-1 in the pancreas 
      of NOD mice following cyclophosphamide administration and during spontaneous 
      diabetes.
PG  - 101-13
AB  - In type 1 diabetes mellitus (T1DM), the processes which control the recruitment 
      of immune cells into pancreatic islets are poorly defined. Complex interactions 
      involving adhesion molecules, chemokines and chemokine receptors may facilitate 
      this process. The chemokine, monocyte chemoattractant protein-1 (MCP-1), 
      previously shown to be important in leukocyte trafficking in other disease 
      systems, may be a key participant in the early influx of blood-borne immune cells 
      into islets during T1DM. In the non-obese diabetic (NOD) mouse, the expression of 
      MCP-1 protein has not been demonstrated. We employed dual-label 
      immunohistochemistry to examine the intra-islet expression, distribution and 
      cellular source of MCP-1 in the NOD mouse following cyclophosphamide 
      administration. NOD mice were treated with cyclophosphamide at day 72-73 and 
      MCP-1 expression studied at days 0, 4, 7, 11 and 14 after treatment and 
      comparisons were made between age-matched NOD mice treated with diluent and 
      non-diabetes-prone CD-1 mice. Pancreatic expression of MCP-1 was also examined in 
      NOD mice at various stages of spontaneous diabetes. In the cyclophosphamide group 
      at day 0, MCP-1 immunolabelling was present in selective peri-islet macrophages 
      but declined at day 4. It increased slightly at day 7 but was more marked from 
      day 11, irrespective of diabetes development. The pattern of MCP-1 expression in 
      macrophages was different over time in both the cyclophosphamide and control 
      groups. In the cyclophosphamide group, there was a change over time with an 
      increase at day 11. In the control group, there was little evidence of change 
      over time. There was no significant difference in the mean percentage of MCP-1 
      positive macrophages between the cyclophosphamide-treated diabetic and 
      non-diabetic mice. During spontaneous diabetes in the NOD mouse, only a few 
      peri-islet MCP-1 cells appeared at day 45. These became more numerous from day 65 
      but were absent at diabetes onset. We speculate that a proportion of early 
      islet-infiltrating macrophages which express MCP-1 may attract additional 
      lymphocytes and macrophages into the early inflamed islets and intensify the 
      process of insulitis.
FAU - Bai, Yan
AU  - Bai Y
AD  - School of Biological Sciences, University of Auckland, Private Bag, 92019, 
      Auckland, New Zealand.
FAU - Robinson, Elizabeth
AU  - Robinson E
FAU - Chai, Ryan
AU  - Chai R
FAU - Ross, Jacqueline M
AU  - Ross JM
FAU - Reddy, Shiva
AU  - Reddy S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060729
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Chemokine CCL2)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Chemokine CCL2/*analysis
MH  - Cyclophosphamide/*administration & dosage
MH  - Diabetes Mellitus/*metabolism/pathology
MH  - Immunohistochemistry
MH  - Inflammation/pathology
MH  - Islets of Langerhans/pathology
MH  - Lymphocytes/physiology
MH  - Macrophages/physiology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Pancreas/*chemistry/*pathology
MH  - Time Factors
EDAT- 2006/10/26 09:00
MHDA- 2008/03/04 09:00
CRDT- 2006/10/26 09:00
PHST- 2006/05/11 00:00 [received]
PHST- 2006/06/28 00:00 [accepted]
PHST- 2006/10/26 09:00 [pubmed]
PHST- 2008/03/04 09:00 [medline]
PHST- 2006/10/26 09:00 [entrez]
AID - 10.1007/s10735-006-9045-6 [doi]
PST - ppublish
SO  - J Mol Histol. 2006 May;37(3-4):101-13. doi: 10.1007/s10735-006-9045-6. Epub 2006 
      Jul 29.