PMID- 17064361
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20091119
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 99
IP  - 5
DP  - 2006 Dec
TI  - Dual regulation of translation initiation and peptide chain elongation during 
      BDNF-induced LTP in vivo: evidence for compartment-specific translation control.
PG  - 1328-37
AB  - Protein synthesis underlying activity-dependent synaptic plasticity is controlled 
      at the level of mRNA translation. We examined the dynamics and spatial regulation 
      of two key translation factors, eukaryotic initiation factor 4E (eIF4E) and 
      elongation factor-2 (eEF2), during long-term potentiation (LTP) induced by local 
      infusion of brain-derived neurotrophic factor (BDNF) into the dentate gyrus of 
      anesthetized rats. BDNF-induced LTP led to rapid, transient phosphorylation of 
      eIF4E and eEF2, and enhanced expression of eIF4E protein in dentate gyrus 
      homogenates. Infusion of the extracellular signal-regulated kinase (ERK) 
      inhibitor U0126 blocked BDNF-LTP and modulation of the translation factor 
      activity and expression. Quantitative immunohistochemical analysis revealed 
      enhanced staining of phospho-eIF4E and total eIF4E in dentate granule cells. The 
      in vitro synaptodendrosome preparation was used to isolate the synaptic effects 
      of BDNF in the dentate gyrus. BDNF treatment of synaptodendrosomes elicited 
      rapid, transient phosphorylation of eIF4E paralleled by enhanced expression of 
      alpha-calcium/calmodulin-dependent protein kinase II. In contrast, BDNF had no 
      effect on eEF2 phosphorylation state in synaptodendrosomes. The results 
      demonstrate rapid ERK-dependent regulation of the initiation and elongation steps 
      of protein synthesis during BDNF-LTP in vivo. Furthermore, the results suggest a 
      compartment-specific regulation in which initiation is selectively enhanced by 
      BDNF at synapses, while both initiation and elongation are modulated at 
      non-synaptic sites.
FAU - Kanhema, Tambudzai
AU  - Kanhema T
AD  - Department of Biomedicine and Bergen Mental Health Research Center, University of 
      Bergen, Bergen, Norway.
FAU - Dagestad, Grethe
AU  - Dagestad G
FAU - Panja, Debabrata
AU  - Panja D
FAU - Tiron, Adrian
AU  - Tiron A
FAU - Messaoudi, Elhoucine
AU  - Messaoudi E
FAU - Havik, Bjarte
AU  - Havik B
FAU - Ying, Shui-Wang
AU  - Ying SW
FAU - Nairn, Angus C
AU  - Nairn AC
FAU - Sonenberg, Nahum
AU  - Sonenberg N
FAU - Bramham, Clive R
AU  - Bramham CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061025
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Eukaryotic Initiation Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Elongation Factor 2)
RN  - 0 (Peptides)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/drug effects/metabolism
MH  - Cell Compartmentation/physiology
MH  - Dendrites/drug effects/metabolism
MH  - Dentate Gyrus/drug effects/*metabolism
MH  - Drug Administration Routes
MH  - Enzyme Inhibitors/pharmacology
MH  - Eukaryotic Initiation Factor-4E/genetics/metabolism
MH  - Eukaryotic Initiation Factors/genetics/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism
MH  - Immunohistochemistry
MH  - Long-Term Potentiation/drug effects/*genetics
MH  - Male
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - Peptide Elongation Factor 2/genetics/metabolism
MH  - Peptides/metabolism
MH  - Phosphorylation
MH  - Presynaptic Terminals/drug effects/metabolism
MH  - Protein Biosynthesis/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synaptic Transmission/drug effects/physiology
MH  - Synaptosomes/drug effects/metabolism
EDAT- 2006/10/27 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/10/27 09:00
PHST- 2006/10/27 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/10/27 09:00 [entrez]
AID - JNC4158 [pii]
AID - 10.1111/j.1471-4159.2006.04158.x [doi]
PST - ppublish
SO  - J Neurochem. 2006 Dec;99(5):1328-37. doi: 10.1111/j.1471-4159.2006.04158.x. Epub 
      2006 Oct 25.