PMID- 17064471
OWN - NLM
STAT- MEDLINE
DCOM- 20100701
LR  - 20161124
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 14
IP  - 10
DP  - 2006 Oct
TI  - [Effects of pentoxifylline on hepatic nuclear factor-kappa B signaling pathway 
      and insulin resistance in nonalcoholic steatohepatitis rats induced by fat-rich 
      diet].
PG  - 762-6
AB  - OBJECTIVE: To explore the effects of pentoxifylline (PTX) on nuclear factor-kappa 
      B (NF-kB) signaling pathway, insulin receptor substrates (IRSs) and glucose 
      transporter 2 (GLUT2) expressions in livers in a rat model of nonalcoholic 
      steatohepatitis (NASH). METHODS: Rats fed a fat-rich diet for 4 weeks were 
      randomly allocated into two groups; the model group rats (n = 12) were fed a 
      high-fat diet alone and the PTX group rats (n = 12) were fed a high-fat diet plus 
      PTX (100 mg x kg(-1)/d(-1)) in drinking water. Meanwhile, rats (n = 6) fed a 
      standard diet from the start served as controls. All the rats were sacrificed at 
      the end of the 24th week. Hepatic NF-kappaB binding activity was measured by 
      electrophoretic mobility shift assay (EMSA). The expression of tumor necrosis 
      factor (TNF) alpha and inhibitor kappaB (IkappaBalpha) proteins in livers were 
      determined by Western blot. Messenger RNA of IRS-1, IRS-2 and GLUT2 expressions 
      were examined by RT-PCR. RESULTS: NF-kappaB binding activity was higher in the 
      model group than that in the controls, while it was lower in the PTX group 
      compared with that in the model group. The expression of TNFalpha protein was 
      markedly increased in the model group (vs. the control group) but decreased in 
      the PTX group (vs. the model group). The expression of IkappaBaalpha protein was 
      decreased in the model group (vs. the control group) but increased in the PTX 
      group (vs. the model group) to a certain extent. IRS-2 mRNA expression was 
      markedly increased in the model group, and significantly decreased in the PTX 
      group when compared with the model group (P less than 0.01). CONCLUSIONS: PTX 
      could influence NF-kappaB signaling pathway and IRS expression in livers of NASH 
      rats, which might be involved in the improvement of hepatic insulin resistance.
FAU - Fan, Jian-Gao
AU  - Fan JG
AD  - Center for Fatty Liver, Shanghai First People's Hospital, Jiaotong University, 
      Shanghai 200080, China.
FAU - Qian, Yan
AU  - Qian Y
FAU - Zheng, Xiao-Ying
AU  - Zheng XY
FAU - Cai, Xiao-Bo
AU  - Cai XB
FAU - Lu, Yuan-Shan
AU  - Lu YS
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Glucose Transporter Type 2)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfkbia protein, rat)
RN  - 0 (Slc2a2 protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Animals
MH  - Fatty Liver/*metabolism
MH  - Glucose Transporter Type 2/metabolism
MH  - I-kappa B Proteins/metabolism
MH  - Insulin Receptor Substrate Proteins/metabolism
MH  - *Insulin Resistance
MH  - Liver/metabolism
MH  - Male
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*metabolism
MH  - Pentoxifylline/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2006/10/27 09:00
MHDA- 2010/07/02 06:00
CRDT- 2006/10/27 09:00
PHST- 2006/10/27 09:00 [pubmed]
PHST- 2010/07/02 06:00 [medline]
PHST- 2006/10/27 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2006 Oct;14(10):762-6.