PMID- 17064765
OWN - NLM
STAT- MEDLINE
DCOM- 20070118
LR  - 20131121
IS  - 0143-4179 (Print)
IS  - 0143-4179 (Linking)
VI  - 40
IP  - 6
DP  - 2006 Dec
TI  - Colocalization of CART peptide with prodynorphin and dopamine D1 receptors in the 
      rat nucleus accumbens.
PG  - 409-15
AB  - CART peptide is a peptidergic neurotransmitter that is expressed in brain regions 
      involved in critical biological processes such as feeding and stress, and in 
      areas associated with drug reward and abuse including the dopamine-rich nucleus 
      accumbens (NAcc), which can be considered part of the basal ganglia. Because CART 
      has been shown to colocalize with substance P, a marker of the basal ganglia 
      direct pathway, we now test for colocalization with other markers of the direct 
      pathway to determine if CART colocalizes with dynorphin and dopamine D1 
      receptors. In the NAcc, CART peptide immunoreactivity (IR) was colocalized with 
      prodynorphin-IR in neurons. Approximately 80.1% of CART-IR cells colocalized with 
      prodynorphin-IR, while only 27.6% of prodynorphin-IR neurons contained CART-IR, 
      suggesting that CART cells are a subset of dynorphin cells. In contrast, only 
      about 25% of CART-IR cell bodies demonstrated dopamine D1 receptor-IR. Because 
      dynorphin and D1 receptors are markers for the basal ganglia direct pathway, from 
      the NAcc to the basal ganglia output nuclei, and because CART significantly 
      colocalizes with these markers, some CART neurons are part of the direct pathway 
      or some comparable pathway in the accumbens. The presence of CART in NAcc neurons 
      and the fact that NAcc projection neurons have extensive local collaterals 
      suggest that CART may have effects in both terminal and cell body regions of the 
      accumbens and may therefore affect information processing in the NAcc by 
      modulating accumbal neurons.
FAU - Hubert, George W
AU  - Hubert GW
AD  - Division of Neuroscience, Yerkes National Research Center, 954 Gatewood Road, 
      Atlanta, GA 30329, USA. ghubert@emory.edu
FAU - Kuhar, Michael J
AU  - Kuhar MJ
LA  - eng
GR  - DA 00418/DA/NIDA NIH HHS/United States
GR  - DA 10732/DA/NIDA NIH HHS/United States
GR  - DA 15040/DA/NIDA NIH HHS/United States
GR  - RR 00165/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061024
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Enkephalins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptides)
RN  - 0 (Protein Precursors)
RN  - 0 (Receptors, Dopamine D1)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
RN  - 93443-35-7 (preproenkephalin)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Animals
MH  - Cocaine/pharmacology
MH  - Enkephalins/*biosynthesis
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Models, Biological
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Neurons/metabolism
MH  - Neuropeptides/chemistry
MH  - Nucleus Accumbens/*metabolism
MH  - Peptides/chemistry
MH  - Protein Precursors/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Dopamine D1/*biosynthesis
MH  - Substance-Related Disorders
EDAT- 2006/10/27 09:00
MHDA- 2007/01/19 09:00
CRDT- 2006/10/27 09:00
PHST- 2006/05/26 00:00 [received]
PHST- 2006/08/01 00:00 [revised]
PHST- 2006/09/02 00:00 [accepted]
PHST- 2006/10/27 09:00 [pubmed]
PHST- 2007/01/19 09:00 [medline]
PHST- 2006/10/27 09:00 [entrez]
AID - S0143-4179(06)00067-9 [pii]
AID - 10.1016/j.npep.2006.09.001 [doi]
PST - ppublish
SO  - Neuropeptides. 2006 Dec;40(6):409-15. doi: 10.1016/j.npep.2006.09.001. Epub 2006 
      Oct 24.