PMID- 17065356
OWN - NLM
STAT- MEDLINE
DCOM- 20070104
LR  - 20220311
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 55
IP  - 11
DP  - 2006 Nov
TI  - Differential expression of matrix metalloproteinase 3 (MMP3) in 
      preadipocytes/stromal vascular cells from nonobese nondiabetic versus obese 
      nondiabetic Pima Indians.
PG  - 3160-5
AB  - Prior microarray studies comparing global gene expression patterns in 
      preadipocytes/stromal vascular cells isolated from nonobese nondiabetic versus 
      obese nondiabetic Pima Indians showed that matrix metalloproteinase 9 (MMP9) is 
      upregulated in obese subjects. The current study targeted analysis of nine 
      additional MMP genes that cluster to a region on chromosome 11q22 that is linked 
      to BMI and percent body fat. Differential-display PCR showed that MMP3 is 
      downregulated in preadipocytes/stromal vascular cells from obese subjects, and 
      real-time PCR showed that MMP3 expression levels are negatively correlated with 
      percent body fat. To determine whether variants within MMP3 are responsible for 
      its altered expression, MMP3 was sequenced, and seven representative variants 
      were genotyped in 1,037 Pima subjects for association analyses. Two variants were 
      associated with both BMI and type 2 diabetes, and two additional variants were 
      associated with type 2 diabetes alone; however, none of these variants were 
      associated with MMP3 expression levels. We propose that the MMP3 pathway is 
      altered in human obesity, but this alteration may be the result of a combination 
      of genetic variation within the MMP3 locus itself, as well as variation in 
      additional factors, either primary or secondary to obesity, that regulate 
      expression of the MMP3 gene.
FAU - Traurig, Michael T
AU  - Traurig MT
AD  - NIDDK, NIH, 445 North 5th St., Phoenix, AZ 85004, USA.
FAU - Permana, Paska A
AU  - Permana PA
FAU - Nair, Saraswathy
AU  - Nair S
FAU - Kobes, Sayuko
AU  - Kobes S
FAU - Bogardus, Clifton
AU  - Bogardus C
FAU - Baier, Leslie J
AU  - Baier LJ
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adipocytes/enzymology
MH  - Adult
MH  - Arizona
MH  - Blood Vessels/enzymology
MH  - Body Mass Index
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11
MH  - Female
MH  - Genetic Variation
MH  - Humans
MH  - Indians, North American/genetics
MH  - Male
MH  - Matrix Metalloproteinase 3/*genetics
MH  - Obesity/genetics
MH  - Polymerase Chain Reaction
MH  - Sex Characteristics
MH  - Stromal Cells/*enzymology
MH  - Thinness/genetics
EDAT- 2006/10/27 09:00
MHDA- 2007/01/05 09:00
CRDT- 2006/10/27 09:00
PHST- 2006/10/27 09:00 [pubmed]
PHST- 2007/01/05 09:00 [medline]
PHST- 2006/10/27 09:00 [entrez]
AID - 55/11/3160 [pii]
AID - 10.2337/db06-0373 [doi]
PST - ppublish
SO  - Diabetes. 2006 Nov;55(11):3160-5. doi: 10.2337/db06-0373.