PMID- 17065789
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20061026
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 115
IP  - 2
DP  - 2006
TI  - Molecular cytogenetic characterization of the 11q13 amplicon in head and neck 
      squamous cell carcinoma.
PG  - 99-106
AB  - Amplification of 11q13 DNA sequences and overexpression of CCND1 are common 
      findings in head and neck squamous cell carcinoma (HNSCC), identified in about 
      30% of the cases. However, little is known about initiation of the amplification 
      and the organization of the amplicon. In order to study the structure of the 
      amplicon in more detail and to learn more about the mechanisms involved in its 
      initiation, prometaphase, metaphase, and anaphase fluorescence in situ 
      hybridization (FISH) with 40 BAC clones spanning a 16-Mb region in chromosome 
      bands 11q12.2 to 11q13.5 was performed in nine HNSCC cell lines with 
      homogeneously staining regions. FISH analysis showed that the size of the 
      amplicon varied among the nine cell lines, the smallest being 2.12 Mb and the 
      largest 8.97 Mb. The smallest overlapping region of amplification was 
      approximately 1.61 Mb, covering the region from BAC 729E14 to BAC 102B19. This 
      region contained several genes previously shown to be amplified and overexpressed 
      in HNSCC, including CCDN1, CTTN, SHANK2, and ORAOV1. The cell lines were also 
      used to study the internal structure of the amplicon. Various patterns of 
      amplified DNA sequences within the amplicon were found among the nine cell lines. 
      Even within the same cell line, different amplicon structures could be found in 
      different cell populations, indicating that the mechanisms involved in the 
      development of the amplicons in HNSCC were more complex than previously assumed. 
      The frequent finding of inverted repeats within the amplicons, however, suggests 
      that breakage-fusion-bridge cycles are important in the initiation, but the fact 
      that such repeats constituted only small parts of the amplicons indicate that 
      they are further rearranged during tumor progression.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Jin, C
AU  - Jin C
AD  - Department of Clinical Genetics, University Hospital, Lund, Sweden. 
      charlotte.jin@med.lu.se
FAU - Jin, Y
AU  - Jin Y
FAU - Gisselsson, D
AU  - Gisselsson D
FAU - Wennerberg, J
AU  - Wennerberg J
FAU - Wah, T S
AU  - Wah TS
FAU - Stromback, B
AU  - Stromback B
FAU - Kwong, Y-L
AU  - Kwong YL
FAU - Mertens, F
AU  - Mertens F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Anaphase
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor/ultrastructure
MH  - Chromosome Banding
MH  - Chromosome Breakage
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Human, Pair 11/*genetics/ultrastructure
MH  - DNA Repair
MH  - DNA, Neoplasm/*genetics
MH  - Disease Progression
MH  - Female
MH  - *Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Metaphase
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2006/10/27 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/10/27 09:00
PHST- 2006/02/20 00:00 [received]
PHST- 2006/03/23 00:00 [accepted]
PHST- 2006/10/27 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/27 09:00 [entrez]
AID - 95228 [pii]
AID - 10.1159/000095228 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2006;115(2):99-106. doi: 10.1159/000095228.