PMID- 17067071
OWN - NLM
STAT- MEDLINE
DCOM- 20080304
LR  - 20061027
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 72
IP  - 1
DP  - 2006 Sep 14
TI  - Mortality and herpesvirus infections of the Pacific oyster Crassostrea gigas in 
      Tomales Bay, California, USA.
PG  - 31-43
AB  - Seed losses of Pacific oysters Crassostrea gigas have been associated with an 
      ostreid herpesvirus-1 (OsHV-1) in Europe, and in 2002, a similar OsHV was 
      detected in Tomales Bay, California, USA. In May of 2003, 5 stocks of seed 
      Pacific oysters were planted at 2 sites (Inner Bay and Outer Bay) in Tomales Bay 
      and monitored for mortality, presence/prevalence of OsHV (using polymerase chain 
      reaction [PCR] and histology), and growth. Temperature (degrees C) and salinity 
      data were collected every half an hour at each site. OsHV was detected at both 
      the Inner and Outer Bay sites on the same sample date and mean temperature 
      predicted OsHV presence (p < 0.005). High levels of mortality occurred 2 wk 
      (Inner Bay site) and 4 wk (Outer Bay site) after OsHV detection. OsHV presence 
      predicted mortality (p = 0.01). Temperature maximums and overall temperature 
      exposure were greater at the Inner Bay site and may explain why mortality 
      affected these oysters sooner than oysters planted at the Outer Bay site. 
      Differences in cumulative mortality were significant among stocks (p < 0.0001), 
      but not between sites (p > 0.05). OsHV prevalence was similar among stocks (p > 
      0.05) and between sites (p > 0.05). No evidence of herpesvirus-induced Cowdry 
      type A nuclear inclusions or other pathogens were observed. Changes in tissue and 
      cellular architecture including dilation of the digestive tubules and nuclear 
      chromatin margination and pycnosis were observed in OsHV-infected oysters, 
      consistent with previously observed OsHV infections. Stocks with smaller oysters 
      had higher mortality rates than those with larger oysters; growth rate did not 
      correlate with mortalities (p > 0.05). Taken together, these data suggest that 
      the OsHV may cause or act in synergy with temperature to kill Pacific oyster seed 
      in Tomales Bay, but further investigation of OsHV etiology in seed oysters is 
      needed.
FAU - Burge, Colleen A
AU  - Burge CA
AD  - School of Aquatic and Fishery Sciences, University of Washington, Box 355020, 
      Seattle, Washington 98195, USA.
FAU - Griffin, Frederick J
AU  - Griffin FJ
FAU - Friedman, Carolyn S
AU  - Friedman CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - California
MH  - Crassostrea/growth & development/*virology
MH  - DNA Primers/chemistry
MH  - Digestive System/pathology
MH  - *Environmental Exposure
MH  - Herpesviridae/*pathogenicity
MH  - Polymerase Chain Reaction/veterinary
MH  - Seawater
MH  - Survival Analysis
MH  - *Temperature
MH  - Time Factors
EDAT- 2006/10/28 09:00
MHDA- 2008/03/05 09:00
CRDT- 2006/10/28 09:00
PHST- 2006/10/28 09:00 [pubmed]
PHST- 2008/03/05 09:00 [medline]
PHST- 2006/10/28 09:00 [entrez]
AID - 10.3354/dao072031 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2006 Sep 14;72(1):31-43. doi: 10.3354/dao072031.