PMID- 17068106
OWN - NLM
STAT- MEDLINE
DCOM- 20070122
LR  - 20061127
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 18
IP  - 12
DP  - 2006 Dec
TI  - IgE-activated mast cells in combination with pro-inflammatory factors induce 
      Th2-promoting dendritic cells.
PG  - 1789-99
AB  - Dendritic cells (DCs) and mast cells (MCs) co-localize in peripheral tissues of 
      antigen entry, i.e. skin and mucosa. Due to the proximity of these two cell 
      types, activation of MCs may affect DC functions. Here, we co-cultured human 
      monocyte-derived DCs with cord blood-derived MCs activated by cross-linking of 
      FcepsilonRI to elucidate the net effect of the whole MC products on DCs. 
      Activated MCs induced maturation of DCs, and potently suppressed IL-12p70 
      production by the DCs. Whereas co-culture of DCs with activated MCs alone did not 
      significantly influence the type of CD4(+) T cell responses induced by the DCs, 
      DCs co-cultured with activated MCs in the presence of pro-inflammatory or 
      T(h)1-inducing factors caused T(h)2 polarization. Although histamine was involved 
      in the induction of DC maturation and T(h)2 polarization by activated MCs, a 
      combinatorial effect of various MC-derived factors, including those acting in a 
      cell contact-dependent manner, was required for the optimal induction of 
      T(h)2-promoting DCs. Furthermore, we demonstrated that clusters of DCs are 
      located closely with MCs in lesions of atopic dermatitis. Collectively, this 
      study suggests that the interaction between DCs and IgE-activated MCs in a 
      pro-inflammatory or even T(h)1-prone environment is instrumental in maintaining 
      and augmenting T(h)2 responses in allergy, and that disruption of the DC-MC 
      interaction may constitute an effective strategy to treat ongoing allergic 
      diseases.
FAU - Kitawaki, Toshio
AU  - Kitawaki T
AD  - Department of Hematology and Oncology, Graduate School of Medicine, Kyoto 
      University, 54 Shogoin Kawara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
FAU - Kadowaki, Norimitsu
AU  - Kadowaki N
FAU - Sugimoto, Naoshi
AU  - Sugimoto N
FAU - Kambe, Naotomo
AU  - Kambe N
FAU - Hori, Toshiyuki
AU  - Hori T
FAU - Miyachi, Yoshiki
AU  - Miyachi Y
FAU - Nakahata, Tatsutoshi
AU  - Nakahata T
FAU - Uchiyama, Takashi
AU  - Uchiyama T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061026
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 187348-17-0 (Interleukin-12)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology
MH  - Dermatitis, Atopic/immunology
MH  - Female
MH  - Fetal Blood
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Inflammation/*immunology
MH  - Interleukin-12/immunology
MH  - Mast Cells/*immunology
MH  - Th2 Cells/cytology/*immunology
EDAT- 2006/10/28 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/10/28 09:00
PHST- 2006/10/28 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/10/28 09:00 [entrez]
AID - dxl113 [pii]
AID - 10.1093/intimm/dxl113 [doi]
PST - ppublish
SO  - Int Immunol. 2006 Dec;18(12):1789-99. doi: 10.1093/intimm/dxl113. Epub 2006 Oct 
      26.