PMID- 17068485
OWN - NLM
STAT- MEDLINE
DCOM- 20070212
LR  - 20221109
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 127
IP  - 2
DP  - 2007 Feb
TI  - Human keratinocytes express functional Toll-like receptor 3, 4, 5, and 9.
PG  - 331-41
AB  - Keratinocytes are continuously in contact with external stimuli and have the 
      capacity to produce several soluble mediators. Pathogen-associated molecular 
      patterns (PAMPs) are recognized, among others, by Toll-like receptors (TLRs). The 
      functional responses of keratinocytes to different PAMPs have not yet been fully 
      established. Here we show that keratinocytes constitutively express TLR1, 2, 3, 
      4, 5, 6, 9, and 10 mRNA, but not TLR7 and 8. Stimulation of keratinocytes with 
      TLR3, 4, 5, and 9 ligands resulted in differential immune-associated responses. 
      Tumor necrosis factor-alpha, CXC chemokine ligand 8 (CXCL8), CCL2, and C 
      chemokine ligand 20 (CCL20) release was enhanced in response to all PAMPs tested, 
      in a time- and dose-dependent manner. Only TLR9 ligand CpG-oligodeoxynucleotides 
      (ODNs) and TLR3 ligand poly-I:C could additionally induce type I IFNs. CCL27 
      production was selectively induced by poly-I:C and flagellin, whereas CXCL9 and 
      CXCL10 were exclusively induced by CpG-ODNs and/or poly-I:C. Upregulation of 
      ICAM-1, HLA-DR, HLA-ABC, FasR, and CD40 was mainly observed in response to 
      poly-I:C, flagellin, and lipopolysaccharide. Furthermore, PAMP triggering 
      resulted in the phosphorylation of phosphorylated-IkappaB alpha and in the 
      nucleus translocation of NF-kappaB p65. Altogether, these findings stress an 
      unexpectedly multifaceted role of keratinocytes in innate immunity as evident by 
      their differential, TLR-mediated responses to PAMPs associated with different 
      classes of pathogens.
FAU - Lebre, Maria C
AU  - Lebre MC
AD  - Department of Cell Biology and Histology, Academic Medical Center, University of 
      Amsterdam, Amsterdam, The Netherlands.
FAU - van der Aar, Angelic M G
AU  - van der Aar AM
FAU - van Baarsen, Lisa
AU  - van Baarsen L
FAU - van Capel, Toni M M
AU  - van Capel TM
FAU - Schuitemaker, Joost H N
AU  - Schuitemaker JH
FAU - Kapsenberg, Martien L
AU  - Kapsenberg ML
FAU - de Jong, Esther C
AU  - de Jong EC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061026
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (CXCL10 protein, human)
RN  - 0 (CXCL9 protein, human)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cytokines)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligonucleotides)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 5)
RN  - 0 (Toll-Like Receptor 9)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Transcription Factor RelA)
RN  - 12777-81-0 (Flagellin)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
CIN - J Invest Dermatol. 2007 Feb;127(2):262-3. PMID: 17228303
MH  - Biological Transport
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Chemokine CXCL10
MH  - Chemokine CXCL9
MH  - Chemokines/metabolism
MH  - Chemokines, CXC/metabolism
MH  - CpG Islands
MH  - Cytokines/metabolism
MH  - Flagellin/pharmacology
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Keratinocytes/*metabolism
MH  - Ligands
MH  - Lipopolysaccharides/pharmacology
MH  - Oligonucleotides/genetics/pharmacology
MH  - Phosphorylation
MH  - Poly I-C/pharmacology
MH  - Protein Isoforms/metabolism
MH  - RNA, Messenger/metabolism
MH  - Tissue Distribution
MH  - Toll-Like Receptor 3/genetics/*metabolism
MH  - Toll-Like Receptor 4/genetics/*metabolism
MH  - Toll-Like Receptor 5/genetics/*metabolism
MH  - Toll-Like Receptor 9/genetics/*metabolism
MH  - Toll-Like Receptors/genetics/metabolism
MH  - Transcription Factor RelA/metabolism
EDAT- 2006/10/28 09:00
MHDA- 2007/02/13 09:00
CRDT- 2006/10/28 09:00
PHST- 2006/10/28 09:00 [pubmed]
PHST- 2007/02/13 09:00 [medline]
PHST- 2006/10/28 09:00 [entrez]
AID - S0022-202X(15)33246-2 [pii]
AID - 10.1038/sj.jid.5700530 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2007 Feb;127(2):331-41. doi: 10.1038/sj.jid.5700530. Epub 2006 
      Oct 26.