PMID- 1706874
OWN - NLM
STAT- MEDLINE
DCOM- 19910429
LR  - 20151119
IS  - 0567-7556 (Print)
IS  - 0567-7556 (Linking)
VI  - 38
DP  - 1990
TI  - Neurofilament expression in bovine chromaffin cells.
PG  - 71-5
AB  - Neurofilament (NF) expression was examined in adult bovine adrenal chromaffin 
      cells by immunocytochemistry using a series of monoclonal antibodies directed 
      against either nonphosphorylated or phosphorylated epitopes of the heavy NF 
      subunit. In situ, this NF subunit was not detected in chromaffin cells. However, 
      chromaffin cells grown in primary culture under standard conditions contained NF 
      proteins, but only in a nonphosphorylated state. Phosphorylation of NFs could be 
      induced under culture conditions favouring the development of a neuronal 
      phenotype--40% of the cells developed neurites within a week while NF 
      phosphorylation occurred later. Phosphorylated NFs were restricted to neurites, 
      unlike nonphosphorylated NFs which were observed in both perikarya and neurites.
FAU - Grant, N J
AU  - Grant NJ
AD  - Centre de Neurochimie du CNRS, Strasbourg, France.
FAU - Aunis, D
AU  - Aunis D
FAU - Langley, O K
AU  - Langley OK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Acta Histochem Suppl
JT  - Acta histochemica. Supplementband
JID - 0061372
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Neurofilament Proteins)
SB  - IM
MH  - Adrenal Medulla/*cytology/ultrastructure
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Axons/ultrastructure
MH  - Cattle
MH  - Cells, Cultured
MH  - Epitopes/analysis
MH  - Fluorescent Antibody Technique
MH  - Immunoenzyme Techniques
MH  - Intermediate Filament Proteins/*analysis/immunology
MH  - Intermediate Filaments/*ultrastructure
MH  - Microscopy, Immunoelectron
MH  - Neurofilament Proteins
MH  - Pituitary Gland/physiology
MH  - Swine
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
PST - ppublish
SO  - Acta Histochem Suppl. 1990;38:71-5.