PMID- 17068816
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20211203
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 25
IP  - 2
DP  - 2007 Feb
TI  - Molecular effects of sodium hyaluronate on the healing of avian supracoracoid 
      tendon tear: according to in situ hybridization and real-time polymerase chain 
      reaction.
PG  - 173-84
AB  - Hyluronic acid (HA) on tissue healing has been controversial. We examined the 
      molecular pharmacology of HA injection at the suture site in an acute model of 
      supracoracoid tendon laceration using chickens, an injury of a nonweight-bearing 
      joint considered similar to the human shoulder. Expression of mRNAs encoding 
      alpha I (I) and alphaI (III) procollagens was localized using in situ 
      hybridization (ISH). Intensities of mRNA expression for alpha I (I) and alpha I 
      (III) procollagens, transforming growth factor-beta1 (TGF- beta1), basic 
      fibroblast growth factor (bFGF), and insulin-like growth factor (IGF) were 
      determined by quantitative reverse transcription-polymerase chain reaction 
      (RT-PCR). Histologically, chickens with HA injection (HA group) showed early 
      restoration of continuity at the laceration site than saline-injection controls 
      (saline-injection group). By ISH, the expression rate of cells at the lesion site 
      that contained alpha I (I) and alpha I (III) procollagen mRNAs were somewhat 
      higher in the HA group than in the saline-injection group. By RT-PCR, the HA- and 
      saline-injection groups showed no significant difference in expression of alpha I 
      (I) and alpha I (III) procollagen mRNA between weeks 1 and 6. The saline 
      -injection group exhibited significant decrease in TGF-beta1 expression between 
      weeks 1 and 3, and in bFGF expression between weeks 1 and 2; however, the HA 
      group showed no such decrease. As for IGF, no difference was appreciable in both 
      groups between weeks 1 and 6. A single injection of HA could cause earlier 
      restoration of continuity at the lacerated site of the supracoracoid tendon.
FAU - Shimpuku, Eiji
AU  - Shimpuku E
AD  - Department of Orthopaedic Surgery, Surgical Science, Tokai University School of 
      Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan. cbt58420@pop21.odn.ne.jp
FAU - Hamada, Kazutoshi
AU  - Hamada K
FAU - Handa, Akiyoshi
AU  - Handa A
FAU - Kobayashi, Kunihiko
AU  - Kobayashi K
FAU - Nakajima, Tomotaka
AU  - Nakajima T
FAU - Yamazaki, Hitoshi
AU  - Yamazaki H
FAU - Nakamura, Masato
AU  - Nakamura M
FAU - Fukuda, Hiroaki
AU  - Fukuda H
FAU - Mochida, Joji
AU  - Mochida J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type I, alpha 1 Chain)
RN  - 0 (RNA, Messenger)
RN  - 0 (Somatomedins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Animals
MH  - Chickens
MH  - Collagen Type I/genetics/metabolism
MH  - Collagen Type I, alpha 1 Chain
MH  - Female
MH  - Fibroblast Growth Factor 2/genetics/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Hyaluronic Acid/*pharmacology
MH  - In Situ Hybridization
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium Chloride/pharmacology
MH  - Somatomedins/genetics/metabolism
MH  - Tendon Injuries/drug therapy/*pathology/physiopathology
MH  - Tendons/*drug effects/pathology/physiopathology
MH  - Transforming Growth Factor beta1/genetics/metabolism
MH  - Wound Healing/*drug effects/physiology
EDAT- 2006/10/28 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/10/28 09:00
PHST- 2006/10/28 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/10/28 09:00 [entrez]
AID - 10.1002/jor.20277 [doi]
PST - ppublish
SO  - J Orthop Res. 2007 Feb;25(2):173-84. doi: 10.1002/jor.20277.