PMID- 17069350
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20190814
IS  - 0024-4201 (Print)
IS  - 0024-4201 (Linking)
VI  - 41
IP  - 7
DP  - 2006 Jul
TI  - Diminished macrophage cholesterol removal rate by the altered HDL metabolism in 
      the Nagase analbuminemic rat.
PG  - 663-8
AB  - Dyslipoproteinemia of the Nagase analbuminemic rat (NAR) is characterized by 
      elevated concentrations of VLDL and LDL attributed to increased rates of liver 
      lipoprotein synthesis. Increased lysophosphatidylcholine (LPC) in NAR HDL has 
      been attributed to high plasma LCAT activity. We show here that, as compared with 
      Sprague-Dawley rats (SDR), NAR plasma triacylglycerol (TAG), total cholesterol 
      (TC), HDL TAG, protein, total phospholipids (PL), LPC, and PS are increased. 
      These alterations rendered the NAR HDL particle more susceptible to the activity 
      of the enzyme hepatic lipoprotein lipase (HL), which otherwise was unaltered in 
      our study. Fractional catabolic rates in blood of the autologous 125I-apoHDL 
      (median and lower quartile values), were, respectively, 0.231 and 1.645 (n = 10) 
      in NAR as compared with 0.140 and 0.109 (n = 10) in SDR (P = 0.012), 
      corresponding to synthesis rates of HDL protein of 89.8 +/- 33.7 mg/d in NAR and 
      17.4 +/- 6.5 mg/d in SDR (P = 0.0122). Furthermore, Swiss mouse macrophage 
      free-cholesterol (FC) efflux rates, measured as the percent [14C]-cholesterol 
      efflux/6 h, were 8.2 +/- 2.3 (n = 9) in NAR HDL and 11.2 +/- 3.2 (n = 10) in SDR 
      HDL (P = 0.03). Therefore, in NAR the modification of the HDL composition slows 
      down the cell FC efflux rate, and together with the increased rate of plasma HDL 
      metabolism influences the reverse cholesterol transport system.
FAU - Catanozi, Sergio
AU  - Catanozi S
AD  - Lipids Laboratory (LIM 10), Faculty of Medical Sciences, University of Sao Paulo, 
      Sao Paulo, Brazil. catanozi@usp.br
FAU - Rocha, Jussara C
AU  - Rocha JC
FAU - Passarelli, Marisa
AU  - Passarelli M
FAU - Mesquita, Carlos H
AU  - Mesquita CH
FAU - Suguiama, Vivian Y
AU  - Suguiama VY
FAU - Guzzo, Maria L
AU  - Guzzo ML
FAU - dos Santos Filho, Antonio
AU  - dos Santos Filho A
FAU - Quintao, Eder C R
AU  - Quintao EC
FAU - Nakandakare, Edna R
AU  - Nakandakare ER
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lipids
JT  - Lipids
JID - 0060450
RN  - 0 (Apolipoproteins)
RN  - 0 (HDL-triglyceride)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Serum Albumin)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Apolipoproteins/blood/*metabolism/pharmacokinetics
MH  - Biological Transport/genetics
MH  - Cholesterol/blood/*metabolism
MH  - Hyperlipoproteinemias/blood/genetics/*metabolism
MH  - Iodine Radioisotopes
MH  - Lipoproteins, HDL/blood/*metabolism
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Rats
MH  - Rats, Mutant Strains
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/*deficiency
MH  - Triglycerides/blood
EDAT- 2006/10/31 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/10/31 09:00
PHST- 2006/10/31 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/10/31 09:00 [entrez]
AID - 10.1007/s11745-006-5017-6 [doi]
PST - ppublish
SO  - Lipids. 2006 Jul;41(7):663-8. doi: 10.1007/s11745-006-5017-6.