PMID- 17069763
OWN - NLM
STAT- MEDLINE
DCOM- 20070119
LR  - 20231213
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 351
IP  - 2
DP  - 2006 Dec 15
TI  - Bidirectional CLOCK/BMAL1-dependent circadian gene regulation by retinoic acid in 
      vitro.
PG  - 387-91
AB  - A central circadian clock located in the suprachiasmatic nucleus (SCN) of the 
      mammalian hypothalamus entrains peripheral clocks through both neural and humoral 
      factors. Although candidates for entrainment factors have been described, their 
      details remain obscure. Here, we screened ligands for nuclear receptors that 
      affect CLOCK/BMAL1-dependent transactivation of the mouse Period1 (mPer1) gene in 
      NIH3T3 cells. We found that retinoic acids (RAs) significantly up-regulate mPer1 
      expression in an E-box-dependent manner. We also found that RAs up-regulate the 
      expression of other E-box-dependent circadian genes such as mPer2, arginine 
      vasopressin (mAVP), and peroxisome proliferator-activated receptor alpha 
      (mPPARalpha). Surprisingly, the effect of RAs on CLOCK/BMAL1 (E-box)-dependent 
      mRNA expression was bidirectional and depended on the presence of exogenous 
      retinoic acid receptor alpha (RARalpha). These results suggest that RAs regulate 
      the CLOCK/BMAL1-dependent transcription of circadian genes in a complex manner.
FAU - Shirai, Hidenori
AU  - Shirai H
AD  - Clock Cell Biology Research Group, Institute for Biological Resources and 
      Functions, National Institute of Advanced Industrial Science and Technology, 
      Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
FAU - Oishi, Katsutaka
AU  - Oishi K
FAU - Ishida, Norio
AU  - Ishida N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061027
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ARNTL Transcription Factors)
RN  - 0 (Bmal1 protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PPAR alpha)
RN  - 0 (Per1 protein, mouse)
RN  - 0 (Per2 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (Rara protein, mouse)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoic Acid Receptor alpha)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 2.3.1.48 (CLOCK Proteins)
RN  - EC 2.3.1.48 (Clock protein, mouse)
SB  - IM
MH  - ARNTL Transcription Factors
MH  - Animals
MH  - Arginine Vasopressin/biosynthesis
MH  - Basic Helix-Loop-Helix Transcription Factors/*metabolism
MH  - CLOCK Proteins
MH  - Cell Cycle Proteins/biosynthesis
MH  - Circadian Rhythm/*physiology
MH  - E-Box Elements/physiology
MH  - Eye Proteins/biosynthesis
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Nuclear Proteins/biosynthesis
MH  - PPAR alpha/biosynthesis
MH  - Period Circadian Proteins
MH  - Receptors, Retinoic Acid/genetics/metabolism
MH  - Retinoic Acid Receptor alpha
MH  - Trans-Activators/*metabolism
MH  - Transcription Factors/biosynthesis
MH  - Transfection
MH  - Tretinoin/pharmacology/*physiology
MH  - Up-Regulation
EDAT- 2006/10/31 09:00
MHDA- 2007/01/20 09:00
CRDT- 2006/10/31 09:00
PHST- 2006/09/22 00:00 [received]
PHST- 2006/10/08 00:00 [accepted]
PHST- 2006/10/31 09:00 [pubmed]
PHST- 2007/01/20 09:00 [medline]
PHST- 2006/10/31 09:00 [entrez]
AID - S0006-291X(06)02269-8 [pii]
AID - 10.1016/j.bbrc.2006.10.031 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Dec 15;351(2):387-91. doi: 
      10.1016/j.bbrc.2006.10.031. Epub 2006 Oct 27.