PMID- 17069782
OWN - NLM
STAT- MEDLINE
DCOM- 20070306
LR  - 20131121
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 377
IP  - 1-2
DP  - 2007 Feb
TI  - Genetic association of interleukin-1beta (-511C/T) and interleukin-1 receptor 
      antagonist (86 bp repeat) polymorphisms with Type 2 diabetes mellitus in North 
      Indians.
PG  - 163-9
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is associated with a subclinical 
      systemic inflammation and development of complications like nephropathy, 
      retinopathy, neuropathy and hypertension. We studied the genetic association of 
      bi-allelic polymorphism (-511C/T) of interleukin (IL)-1beta and 86 bp variable 
      number tandem repeat (VNTR) polymorphism of natural receptor antagonist (IL-1RN) 
      with T2DM and associated complications in North Indians. METHODS: We genotyped 
      200 patients with T2DM and 223 healthy control subjects for IL-1beta (-511C/T) by 
      PCR-RFLP. Genotyping of IL-1RN (VNTR) polymorphism was determined by gel 
      electrophoresis after PCR amplification. RESULTS: Interleukin-1beta (-511C/T) and 
      IL-1RN (VNTR) polymorphisms were significantly associated with T2DM. IL-1beta 
      -511T, IL-1RN*2 and IL-1RN*3 alleles were associated with high risk of T2DM 
      whereas; individuals with IL-1beta -511C and IL-1RN*1 alleles were at low risk. 
      Haplotype frequency analysis showed that T2 (IL-1beta -511T/IL-1RN*2) haplotype 
      was associated with the high risk (p=0.000; OR=2.4, 95% CI 1.68-3.34) and C1 
      (IL-1beta -511C/IL-1RN*1) haplotype showed low risk (p=0.000; OR=0.38, 95% CI 
      0.27-0.53). Further, CT, TT genotypes of IL-1beta (-511C/T) and 1/2 genotype of 
      IL-1RN (VNTR) were found to be associated with risk of complications particularly 
      with nephropathy in T2DM. CONCLUSION: The IL-1beta (-511C/T) and IL-1RN (VNTR) 
      polymorphisms are significantly associated with increased risk of T2DM as well as 
      associated complications in North Indians.
FAU - Achyut, B R
AU  - Achyut BR
AD  - Sanjay Gandhi Postgraduate Institute of Medical Sciences Lucknow-226014, India.
FAU - Srivastava, Akanksha
AU  - Srivastava A
FAU - Bhattacharya, Sandeep
AU  - Bhattacharya S
FAU - Mittal, Balraj
AU  - Mittal B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061027
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Interleukin 1 Receptor Antagonist Protein)
RN  - 0 (Interleukin-1beta)
RN  - 5CSZ8459RP (Cytidine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Cytidine/*genetics
MH  - Diabetes Mellitus, Type 2/complications/*genetics/pathology
MH  - Female
MH  - Genotype
MH  - Humans
MH  - India
MH  - Interleukin 1 Receptor Antagonist Protein/*genetics
MH  - Interleukin-1beta/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic/*genetics
MH  - Risk Factors
MH  - Thymidine/*genetics
EDAT- 2006/10/31 09:00
MHDA- 2007/03/07 09:00
CRDT- 2006/10/31 09:00
PHST- 2006/07/10 00:00 [received]
PHST- 2006/09/18 00:00 [revised]
PHST- 2006/09/18 00:00 [accepted]
PHST- 2006/10/31 09:00 [pubmed]
PHST- 2007/03/07 09:00 [medline]
PHST- 2006/10/31 09:00 [entrez]
AID - S0009-8981(06)00616-4 [pii]
AID - 10.1016/j.cca.2006.09.012 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2007 Feb;377(1-2):163-9. doi: 10.1016/j.cca.2006.09.012. Epub 
      2006 Oct 27.