PMID- 17069927
OWN - NLM
STAT- MEDLINE
DCOM- 20070307
LR  - 20141120
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 46
IP  - 1
DP  - 2007 Jan
TI  - Fibrogenic cell fate during fibrotic tissue remodelling observed in rat and human 
      cultured liver slices.
PG  - 142-50
AB  - BACKGROUND/AIMS: Fibrotic liver remodelling was studied in culture of 
      precision-cut liver slices (PCLS) derived from fibrotic liver. METHODS: Fibrosis 
      was induced in rats by carbon tetrachloride (CCl4) treatment or bile duct 
      ligation. Human fibrotic livers were also used. PCLS were cultured for 6, 24, 48, 
      or 72 h, and the expression of alpha-smooth muscle (SM) actin, platelet-derived 
      growth factor (PDGF) receptor-beta, and active caspase 3 was studied by 
      immunohistochemistry. RESULTS: Before culture, in CCl4-treated or bile duct 
      ligated animals, fibrosis was observed around centrolobular veins, or in portal 
      zones, respectively. In PCLS derived from CCl4-treated animals, alpha-SM actin 
      expression disappeared after 24h in culture while PDGF receptor-beta expression 
      decreased progressively after 48 h. These changes were observed in absence of 
      massive apoptosis. In PCLS derived from bile duct ligated animals, both alpha-SM 
      actin and PDGF receptor-beta expression decreased after 48 h in culture with a 
      massive apoptosis. In PCLS derived from human fibrotic livers, alpha-SM actin 
      expression was dramatically reduced after 48 h in culture. CONCLUSIONS: After 
      CCl4 treatment, a proportion of myofibroblasts derived from hepatic stellate 
      cells seems to dedifferentiate while in bile duct ligation model, myofibroblasts 
      derived from portal fibroblasts disappear by apoptosis, underlining the relevance 
      of this model to evaluate the mechanisms involved in fibrotic liver remodelling.
FAU - Guyot, Christelle
AU  - Guyot C
AD  - INSERM, E0362, Universite Victor Segalen Bordeaux 2, Bordeaux F-33076, France.
FAU - Combe, Chantal
AU  - Combe C
FAU - Balabaud, Charles
AU  - Balabaud C
FAU - Bioulac-Sage, Paulette
AU  - Bioulac-Sage P
FAU - Desmouliere, Alexis
AU  - Desmouliere A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061004
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Actins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (platelet-derived growth factor A)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Actins/metabolism
MH  - Aged
MH  - Animals
MH  - Carbon Tetrachloride/toxicity
MH  - Extracellular Matrix/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Liver Cirrhosis/etiology/metabolism/*pathology
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Phenotype
MH  - Platelet-Derived Growth Factor/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Culture Techniques/methods
EDAT- 2006/10/31 09:00
MHDA- 2007/03/08 09:00
CRDT- 2006/10/31 09:00
PHST- 2006/03/18 00:00 [received]
PHST- 2006/08/24 00:00 [revised]
PHST- 2006/08/27 00:00 [accepted]
PHST- 2006/10/31 09:00 [pubmed]
PHST- 2007/03/08 09:00 [medline]
PHST- 2006/10/31 09:00 [entrez]
AID - S0168-8278(06)00498-3 [pii]
AID - 10.1016/j.jhep.2006.08.013 [doi]
PST - ppublish
SO  - J Hepatol. 2007 Jan;46(1):142-50. doi: 10.1016/j.jhep.2006.08.013. Epub 2006 Oct 
      4.