PMID- 17070934
OWN - NLM
STAT- MEDLINE
DCOM- 20070221
LR  - 20061218
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 115
IP  - 1-2
DP  - 2007 Jan 15
TI  - IL-13 replaces IL-4 in development of monocyte derived dendritic cells (MoDC) of 
      swine.
PG  - 56-67
AB  - Dendritic cells (DCs) are a critical aspect of innate immune responses in 
      addition to initiating adaptive immunity. In vitro generation of monocyte derived 
      dendritic cells (MoDC) by culturing cells in IL-4 and granulocyte/macrophage 
      colony stimulating factor (GM-CSF) has been reported for multiple species 
      including swine. However, IL-4 is not a prominent cytokine detected in the 
      periphery of common breeds of swine such as Yorkshire pigs. In this study, we 
      report the generation and characterization of porcine MoDC in vitro using porcine 
      IL-13 and porcine GM-CSF. These cells have the predicted expression of Class II 
      MHC and T cell costimulatory molecules, phagocytic capacity and the ability to 
      process and present antigen. Critically, porcine IL-13/GM-CSF MoDC have the 
      unique ability to stimulate a primary mixed lymphocyte response in vitro. The 
      type I interferon response of these MoDC to poly I:C (TLR3 ligand), LPS (TLR4 
      ligand) and CpG (TLR9 ligand) was tested. Of these TLR agonists, LPS or CpG did 
      not stimulate induction of type I interferons, but a strong response was observed 
      to poly I:C. This analysis shows that the generation of MoDCs in IL-13 yields 
      cells of equivalent phenotype and function as IL-4 generated DC. However, for 
      swine, in vitro generation of MoDC in IL-13 is likely to induce a more 
      physiological cell population to study given expression of IL-4 is lacking in the 
      periphery of these animals.
FAU - Bautista, Elida M
AU  - Bautista EM
AD  - Foot-and-Mouth Disease Research Unit, Plum Island Animal Disease Center, ARS, 
      USDA, Greenport, NY 11944, USA.
FAU - Nfon, Charles
AU  - Nfon C
FAU - Ferman, Geoffery S
AU  - Ferman GS
FAU - Golde, William T
AU  - Golde WT
LA  - eng
PT  - Journal Article
DEP - 20061027
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Interferon Type I)
RN  - 0 (Interleukin-13)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Differentiation
MH  - Dendritic Cells/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/physiology
MH  - Interferon Type I/biosynthesis
MH  - Interleukin-13/*physiology
MH  - Interleukin-4/*physiology
MH  - Lymphocyte Culture Test, Mixed
MH  - Molecular Sequence Data
MH  - Monocytes/*cytology
MH  - Phagocytosis
MH  - Swine
EDAT- 2006/10/31 09:00
MHDA- 2007/02/22 09:00
CRDT- 2006/10/31 09:00
PHST- 2006/06/01 00:00 [received]
PHST- 2006/07/25 00:00 [revised]
PHST- 2006/09/07 00:00 [accepted]
PHST- 2006/10/31 09:00 [pubmed]
PHST- 2007/02/22 09:00 [medline]
PHST- 2006/10/31 09:00 [entrez]
AID - S0165-2427(06)00249-2 [pii]
AID - 10.1016/j.vetimm.2006.09.010 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2007 Jan 15;115(1-2):56-67. doi: 
      10.1016/j.vetimm.2006.09.010. Epub 2006 Oct 27.