PMID- 17071983
OWN - NLM
STAT- MEDLINE
DCOM- 20070103
LR  - 20061030
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 349
DP  - 2006
TI  - Application of yeast artificial chromosomes in fluorescence in situ 
      hybridization.
PG  - 175-86
AB  - In addition to the well-known applications of yeast artificial chromosomes (YACs) 
      in classical molecular genetics, they also are used for molecular cytogenetic 
      studies. YACs, as well as other locus-specific probes like DNA, plasmids, 
      cosmids, P1-clones, or bacterial artificial chromosomes can be labeled with 
      fluorochromes and applied in fluorescence in situ hybridization (FISH) 
      experiments. Various applications are possible, such as gene mapping, FISH 
      banding, determination of chromosomal breakpoints, characterization of derivative 
      chromosomes, studies on the interphase architecture, or karyotypic evolution 
      studies. This chapter outlines the basic principle of how YACs can be hybridized 
      in situ on chromosome preparations. Moreover, an overview is given on possible 
      questions to be processed using YACs as FISH probes.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Institute of Human Genetics and Anthropology, Friedrich-Schiller-University Jena, 
      Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Mapping
MH  - *Chromosomes, Artificial, Yeast
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
EDAT- 2006/10/31 09:00
MHDA- 2007/01/04 09:00
CRDT- 2006/10/31 09:00
PHST- 2006/10/31 09:00 [pubmed]
PHST- 2007/01/04 09:00 [medline]
PHST- 2006/10/31 09:00 [entrez]
AID - 1-59745-158-4:175 [pii]
AID - 10.1385/1-59745-158-4:175 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;349:175-86. doi: 10.1385/1-59745-158-4:175.