PMID- 17074039 OWN - NLM STAT- MEDLINE DCOM- 20061221 LR - 20091119 IS - 0953-816X (Print) IS - 0953-816X (Linking) VI - 24 IP - 8 DP - 2006 Oct TI - BDNF occludes GABA receptor-mediated inhibition of GABA release in rat hippocampal CA1 pyramidal neurons. PG - 2135-44 AB - During the development of the rat hippocampus, both gamma-aminobutyric acid (GABA)(B) autoreceptors and brain-derived neurotrophic factor (BDNF) play important roles in the formation of GABAergic synapses as well as in the GABA(A) receptor-mediated transmissions. While a number of studies have reported rapid effects of BDNF on GABA(A) receptor-mediated responses, the interactions between GABA(B) autoreceptors and BDNF are less clear. Using conventional whole-cell patch-clamp recordings, we demonstrated here that BDNF significantly occludes baclofen-induced suppression of GABA(A) receptor-mediated transmissions in each of the preparations including hippocampal slices prepared from P14 rats, hippocampal CA1 pyramidal neurons isolated from P14 and P21 rats, and cultured hippocampal pyramidal neurons. This effect of BDNF was rapid and reversible, and was mediated via the activation of presynaptic TrkB receptor tyrosine kinases, and subsequent activation of phospholipase C and protein kinase C. On the contrary, in hippocampal CA1 pyramidal neurons isolated from P7 rats, BDNF failed to occlude the GABA(B) receptor-mediated inhibition of GABA release. Thus, the ability of BDNF to occlude the GABA(B) receptor-mediated inhibition of GABA release develops between P7 and P14. This demonstrates a novel aspect of the effects of BDNF on inhibitory transmissions in rat hippocampus, which may have some functional roles in the induction of developmental plasticity and/or pathophysiology of epilepsy. FAU - Mizoguchi, Yoshito AU - Mizoguchi Y AD - Division of Homeostatic Development, Department of Developmental Physiology, National Institute of Physiological Sciences, 38 Myodaiji, Okazaki 444-8585 Japan. FAU - Kitamura, Akihiko AU - Kitamura A FAU - Wake, Hiroaki AU - Wake H FAU - Ishibashi, Hitoshi AU - Ishibashi H FAU - Watanabe, Miho AU - Watanabe M FAU - Nishimaki, Takuya AU - Nishimaki T FAU - Nabekura, Junichi AU - Nabekura J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - France TA - Eur J Neurosci JT - The European journal of neuroscience JID - 8918110 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (GABA Agonists) RN - 0 (GABA Antagonists) RN - 0 (Receptors, GABA-B) RN - 37558-16-0 (Phorbol 12,13-Dibutyrate) RN - 56-12-2 (gamma-Aminobutyric Acid) RN - EC 2.7.10.1 (Receptor, trkB) RN - EC 2.7.11.13 (Protein Kinase C) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cells, Cultured MH - Electrophysiology MH - Excitatory Postsynaptic Potentials/drug effects MH - GABA Agonists/pharmacology MH - GABA Antagonists/pharmacology MH - Hippocampus/cytology/drug effects/*metabolism MH - Patch-Clamp Techniques MH - Phorbol 12,13-Dibutyrate/pharmacology MH - Protein Kinase C/metabolism MH - Pyramidal Cells/drug effects/*metabolism MH - Rats MH - Rats, Wistar MH - Receptor, trkB/metabolism MH - Receptors, GABA-B/*drug effects MH - Signal Transduction/drug effects MH - gamma-Aminobutyric Acid/*metabolism EDAT- 2006/11/01 09:00 MHDA- 2006/12/22 09:00 CRDT- 2006/11/01 09:00 PHST- 2006/11/01 09:00 [pubmed] PHST- 2006/12/22 09:00 [medline] PHST- 2006/11/01 09:00 [entrez] AID - EJN5092 [pii] AID - 10.1111/j.1460-9568.2006.05092.x [doi] PST - ppublish SO - Eur J Neurosci. 2006 Oct;24(8):2135-44. doi: 10.1111/j.1460-9568.2006.05092.x.