PMID- 17074587
OWN - NLM
STAT- MEDLINE
DCOM- 20061215
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 171
IP  - 1
DP  - 2006 Nov
TI  - A case of acute myelogenous leukemia with MLL-AF10 fusion caused by insertion of 
      5' MLL into 10p12, with concurrent 3' MLL deletion.
PG  - 24-30
AB  - Structural abnormalities involving the mixed-lineage leukemia (MLL) gene on 11q23 
      have been associated with hematological malignancies. The rearrangement of MLL 
      occurs during translocations and insertions involving a variety of genes on the 
      partner chromosome. We report a rare case of acute myelogenous leukemia (AML-M2) 
      with 11q23 abnormalities. Fluorescence in situ hybridization (FISH) using a 
      commercial dual-color MLL probe detected an atypical signal pattern: one fusion 
      signal, two green signals smaller than those usually detected, and no orange 
      signals. Spectral karyotyping (SKY) analysis indicated that one green signal was 
      detected on the short arm of derivative chromosome 10, and the other green signal 
      on the long arm of a derivative chromosome 11, on which no orange signal was 
      detected. A long-distance inverse polymerase chain reaction (LDI-PCR) identified 
      the fusion partner gene, in which intron 6 of MLL was fused with intron 8 of AF10 
      on 10p12 in the 5' to 3' direction. Our observations indicated that the MLL-AF10 
      fusion gene resulted from the insertion of part of the region that included the 
      5' MLL insertion into 10p12; this was concurrent with the deletion of 3' MLL.
FAU - Matsuda, Kazuyuki
AU  - Matsuda K
AD  - Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asashi, 
      Matsumoto, Japan.
FAU - Hidaka, Eiko
AU  - Hidaka E
FAU - Ishida, Fumihiro
AU  - Ishida F
FAU - Yamauchi, Kazuyoshi
AU  - Yamauchi K
FAU - Makishima, Hideki
AU  - Makishima H
FAU - Ito, Toshiro
AU  - Ito T
FAU - Suzuki, Takefumi
AU  - Suzuki T
FAU - Imagawa, Eri
AU  - Imagawa E
FAU - Sano, Kenji
AU  - Sano K
FAU - Katsuyama, Tsutomu
AU  - Katsuyama T
FAU - Ota, Hiroyoshi
AU  - Ota H
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (KMT2A protein, human)
RN  - 0 (MLL-AF10 fusion protein, human)
RN  - 0 (MLLT10 protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Breakage
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - *Gene Deletion
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Leukemia, Myeloid/genetics/*pathology
MH  - Male
MH  - Mutagenesis, Insertional/genetics
MH  - Myeloid-Lymphoid Leukemia Protein/chemistry/*genetics
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Sequence Analysis, DNA
MH  - Spectral Karyotyping/methods
MH  - Transcription Factors/genetics
EDAT- 2006/11/01 09:00
MHDA- 2006/12/16 09:00
CRDT- 2006/11/01 09:00
PHST- 2006/03/21 00:00 [received]
PHST- 2006/06/01 00:00 [revised]
PHST- 2006/06/06 00:00 [accepted]
PHST- 2006/11/01 09:00 [pubmed]
PHST- 2006/12/16 09:00 [medline]
PHST- 2006/11/01 09:00 [entrez]
AID - S0165-4608(06)00410-9 [pii]
AID - 10.1016/j.cancergencyto.2006.06.013 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2006 Nov;171(1):24-30. doi: 
      10.1016/j.cancergencyto.2006.06.013.