PMID- 17074926
OWN - NLM
STAT- MEDLINE
DCOM- 20070716
LR  - 20210217
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 48
IP  - 1
DP  - 2007 Jan
TI  - Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation 
      by human leukocyte antigen class I molecules.
PG  - 127-38
AB  - Dietary supplementation with polyunsaturated fatty acids (PUFAs) has 
      immunosuppressive effects; however, the molecular targets of PUFAs and their mode 
      of action remain unclear. One possible target is antigen presentation to T cells 
      through the human leukocyte antigen (HLA) class I pathway. Here we show that 
      incorporation of PUFAs lowers target cell susceptibility to lysis by effector T 
      cells. Treatment of B lymphoblast targets with the omega-6 PUFA arachidonic acid 
      (AA) or omega-3 docosahexaenoic acid lowered their susceptibility to lysis by 
      alloreactive CD8+ T cells by approximately 20-25%. HLA class I surface levels and 
      their rate of endoplasmic reticulum (ER)-Golgi traffic were also reduced by PUFA 
      treatment. Calibration experiments showed that the approximately 15% reduction in 
      surface HLA I was not sufficient to completely account for the decreased lysis. 
      However, PUFAs significantly lowered antigen-presenting cell-T cell conjugate 
      formation, by approximately 30-40%. Taken together, our data show for the first 
      time that an omega-6 and an omega-3 PUFA affect the HLA class I pathway of B 
      lymphoblasts. Our findings suggest that elimination of self- and pathogen-derived 
      peptides by effectors may be compromised by dietary PUFA supplementation. In 
      addition, PUFA-mediated changes in ER-Golgi trafficking point to a new area of 
      PUFA modulation of immune responses.
FAU - Shaikh, Saame Raza
AU  - Shaikh SR
AD  - Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA. 
      saameshaikh@gmail.com
FAU - Edidin, Michael
AU  - Edidin M
LA  - eng
GR  - 5T32 AI-07247/AI/NIAID NIH HHS/United States
GR  - AI-14584/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061030
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Phospholipids)
RN  - FZ989GH94E (Povidone)
SB  - IM
MH  - Antigen-Presenting Cells/drug effects/*immunology
MH  - B-Lymphocytes/drug effects/*immunology
MH  - Cell Line
MH  - Fatty Acids, Unsaturated/*pharmacology
MH  - Genes, Reporter
MH  - HLA-A2 Antigen/genetics/immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Phospholipids/pharmacology
MH  - Povidone/pharmacology
MH  - Transfection
EDAT- 2006/11/01 09:00
MHDA- 2007/07/17 09:00
CRDT- 2006/11/01 09:00
PHST- 2006/11/01 09:00 [pubmed]
PHST- 2007/07/17 09:00 [medline]
PHST- 2006/11/01 09:00 [entrez]
AID - S0022-2275(20)43643-0 [pii]
AID - 10.1194/jlr.M600365-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2007 Jan;48(1):127-38. doi: 10.1194/jlr.M600365-JLR200. Epub 2006 
      Oct 30.