PMID- 17077309
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20120215
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 20
IP  - 13
DP  - 2006 Nov
TI  - The spleen as a target in severe acute respiratory syndrome.
PG  - 2321-8
AB  - It has been proposed that immune injury is the central mechanism of pathogenesis 
      of the infectious disease, severe acute respiratory syndrome (SARS). To gain a 
      better understanding of immune injury in the spleen, we investigated the number 
      and distribution of various immune cell types in the spleens of SARS patients. We 
      performed autopsies on six confirmed SARS cases, with six normal subjects as 
      controls; spleen samples from these autopsies were examined with hematoxylin and 
      eosin (H&E) sections, in situ hybridization for SARS virus genomic sequences, and 
      immunohistochemistry with seven monoclonal antibodies to five cell types. The 
      number and distribution of these cells were measured and analyzed using an image 
      analysis system. SARS genomic sequences were detected in all SARS spleens. The 
      SARS spleens all had severe damage to the white pulp and showed an alteration of 
      the normal distribution of various cell types. Immunocytes in the red pulp were 
      decreased by 68.0-90.7% except for CD68+ macrophages and human leukocyte antigen 
      (HLA)-DR positive antigen-presenting cells (APC), which were decreased to a 
      lesser degree. On average, CD68+ macrophages were increased in size by 2.21-fold. 
      We hypothesize that the collapse of the splenic immune system plays a key role in 
      the clinical outcome of these patients.
FAU - Zhan, Jun
AU  - Zhan J
AD  - Department of Histology and Embryology, Peking University Health Science Center, 
      Beijing, China.
FAU - Deng, Ruishu
AU  - Deng R
FAU - Tang, Junmin
AU  - Tang J
FAU - Zhang, Bo
AU  - Zhang B
FAU - Tang, Yan
AU  - Tang Y
FAU - Wang, Jeffrey K
AU  - Wang JK
FAU - Li, Feng
AU  - Li F
FAU - Anderson, Virginia M
AU  - Anderson VM
FAU - McNutt, Michael A
AU  - McNutt MA
FAU - Gu, Jiang
AU  - Gu J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Antigens, CD)
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Autopsy
MH  - Base Sequence
MH  - Coronavirus/genetics/isolation & purification
MH  - DNA Primers
MH  - Genome, Viral
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Severe Acute Respiratory Syndrome/*pathology
MH  - Spleen/*pathology
EDAT- 2006/11/02 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/11/02 09:00
PHST- 2006/11/02 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/11/02 09:00 [entrez]
AID - 20/13/2321 [pii]
AID - 10.1096/fj.06-6324com [doi]
PST - ppublish
SO  - FASEB J. 2006 Nov;20(13):2321-8. doi: 10.1096/fj.06-6324com.