PMID- 17079045
OWN - NLM
STAT- MEDLINE
DCOM- 20070927
LR  - 20131121
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 124
IP  - 1-2
DP  - 2007 Mar
TI  - Alpha- and betapapillomavirus E6/E7 genes differentially modulate 
      pro-inflammatory gene expression.
PG  - 220-5
AB  - Keratinocytes, the target cell of human papillomavirus (HPV) infection, can 
      produce numerous cytokines and pro-inflammatory molecules which are important for 
      the generation of an effective immune response. How this biological response, 
      which involves the tumor stroma, is affected by the HPV oncoproteins within the 
      epithelial cell itself is not clear. Here it is shown that oncoproteins of 
      different HPV genotypes (alpha- versus beta-HPV genus) alter the expression of 
      pro-inflammatory molecules in early passage primary human keratinocytes and the 
      immortalized cell line HaCaT. HPV5 E6/E7 oncoproteins significantly induced 
      interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1) and 
      intercellular adhesion molecule-1 (ICAM-1) expression. By contrast, the same 
      molecules were down-regulated or not modulated in HPV16 E6/E7 transduced 
      keratinocytes. Interestingly, HPV38 oncoproteins expression resulted in a lower 
      induction of pro-inflammatory molecules, resembling the behavior displayed by the 
      mucosal carcinogenic HPV16. Finally, inducible nitric oxide synthase (iNOS) 
      expression levels and nitric oxide (NO) production were induced at similar levels 
      by all the HPV genotypes tested. These results further emphasize the different 
      biological activities among HPV genotypes, and offer new insights into 
      HPV-associated skin diseases.
FAU - De Andrea, Marco
AU  - De Andrea M
AD  - Department of Public Health and Microbiology, Medical School of Torino,Via 
      Santena 9, 10126 Torino, Italy.
FAU - Mondini, Michele
AU  - Mondini M
FAU - Azzimonti, Barbara
AU  - Azzimonti B
FAU - Dell'Oste, Valentina
AU  - Dell'Oste V
FAU - Germano, Serena
AU  - Germano S
FAU - Gaudino, Giovanni
AU  - Gaudino G
FAU - Musso, Tiziana
AU  - Musso T
FAU - Landolfo, Santo
AU  - Landolfo S
FAU - Gariglio, Marisa
AU  - Gariglio M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061031
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (E6 protein, human papillomavirus type 5)
RN  - 0 (Interleukin-8)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus E7 Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (oncogene protein E7, Human papillomavirus type 16)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Alphapapillomavirus/genetics/*immunology
MH  - Betapapillomavirus/genetics/*immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Human papillomavirus 16/genetics/immunology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/biosynthesis
MH  - Interleukin-8/biosynthesis
MH  - Keratinocytes/immunology/*virology
MH  - Nitric Oxide/biosynthesis
MH  - Nitric Oxide Synthase/biosynthesis
MH  - Oncogene Proteins, Viral/genetics/*immunology
MH  - Papillomavirus E7 Proteins
MH  - Repressor Proteins/genetics/immunology
EDAT- 2006/11/03 09:00
MHDA- 2007/09/28 09:00
CRDT- 2006/11/03 09:00
PHST- 2006/06/19 00:00 [received]
PHST- 2006/09/28 00:00 [revised]
PHST- 2006/09/28 00:00 [accepted]
PHST- 2006/11/03 09:00 [pubmed]
PHST- 2007/09/28 09:00 [medline]
PHST- 2006/11/03 09:00 [entrez]
AID - S0168-1702(06)00293-0 [pii]
AID - 10.1016/j.virusres.2006.09.010 [doi]
PST - ppublish
SO  - Virus Res. 2007 Mar;124(1-2):220-5. doi: 10.1016/j.virusres.2006.09.010. Epub 
      2006 Oct 31.