PMID- 17079495
OWN - NLM
STAT- MEDLINE
DCOM- 20070301
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 45
IP  - 1
DP  - 2007 Jan
TI  - Genetic profiling of the oral microbiota associated with severe early-childhood 
      caries.
PG  - 81-7
AB  - The determination of the composition of the microbial community in the oral 
      cavity is usually based on cultivation methods; however, nearly half of the 
      bacteria in the saliva and the dental plaque are not cultivable. In this study, 
      we evaluated the difference in oral microbial diversity between children with 
      severe early-childhood caries (S-ECC) and caries-free (CF) controls by means of a 
      cultivation-independent approach called denaturing gradient gel electrophoresis 
      (DGGE). Pooled dental plaque samples were collected from 20 children aged 2 to 8 
      years. Total microbial genomic DNA was isolated from those subjects, and a 
      portion of the 16S rRNA gene locus was PCR amplified by using universal primers. 
      We observed that the mean species richness of the bacterial population was 
      greater in the CF children (n = 12) (42 +/- 3.7) than in the S-ECC children (n = 
      8) (35 +/- 4.3); the difference was statistically significant (P = 0.005). The 
      overall diversity of plaque samples as measured by the Shannon index was 3.5 for 
      the S-ECC group and 3.7 for the CF group (P = 0.004). Differences in DGGE 
      profiles were distinguished on the basis of a cluster analysis. Sequence analysis 
      of excised DGGE bands consisted of 2.7 phylotypes, on average. After adjusting 
      for the number of observed bands, we estimated that the S-ECC group exhibited 
      94.5 total phylotypes and that the CF group exhibited 113.4. These results 
      suggest that the microbial diversity and complexity of the microbial biota in 
      dental plaque are significantly less in S-ECC children than in CF children.
FAU - Li, Y
AU  - Li Y
AD  - Department of Basic Science and Craniofacial Biology, New York University College 
      of Dentistry, New York, NY 10010, USA. yihong.li@nyu.edu
FAU - Ge, Y
AU  - Ge Y
FAU - Saxena, D
AU  - Saxena D
FAU - Caufield, P W
AU  - Caufield PW
LA  - eng
GR  - R01 DE013937/DE/NIDCR NIH HHS/United States
GR  - DE13937/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061101
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Bacterial Typing Techniques
MH  - Child
MH  - Child, Preschool
MH  - Cluster Analysis
MH  - DNA, Bacterial/*analysis/isolation & purification
MH  - Dental Caries/*microbiology
MH  - Dental Plaque/*microbiology
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Female
MH  - Gram-Negative Bacteria/classification/genetics/*isolation & purification
MH  - Gram-Positive Bacteria/classification/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
PMC - PMC1828962
EDAT- 2006/11/03 09:00
MHDA- 2007/03/03 09:00
PMCR- 2007/05/01
CRDT- 2006/11/03 09:00
PHST- 2006/11/03 09:00 [pubmed]
PHST- 2007/03/03 09:00 [medline]
PHST- 2006/11/03 09:00 [entrez]
PHST- 2007/05/01 00:00 [pmc-release]
AID - JCM.01622-06 [pii]
AID - 1622-06 [pii]
AID - 10.1128/JCM.01622-06 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2007 Jan;45(1):81-7. doi: 10.1128/JCM.01622-06. Epub 2006 Nov 
      1.